DOT1L
DOT1L (DOT1 like histone lysine methyltransferase, also known as KMT4, DOT1 and KIAA1814) is a histone methyltransferase that methylates lysine-79 of histone H3. It acts on nucleosomal substrates and contributes to transcriptional repression at specific promoters. Browse Assay Genie's current range of DOT1L research reagents below, or read on for the biology behind the target.
DOT1L is a histone methyltransferase that methylates 'Lys-79' of histone H3 (H3K79). It shows a clear preference for histones assembled in nucleosomes over free histones, and it is reported to be inactive against free core histones while showing significant activity against nucleosomes. The protein also binds DNA. In one documented example, DOT1L works together with MLLT3/AF9 in the transcriptional repression of SCNN1A, following H3K79 hypermethylation at the promoter.
The DOT1L gene encodes a protein that belongs to the DOT1 family within the class I-like SAM-binding methyltransferase superfamily. HGNC places it among the histone lysine methyltransferases and the 7BS protein lysine methyltransferases, and it carries the EC number 2.1.1.360. Its subcellular location is annotated as nucleus and cytoplasm. The mouse ortholog is Dot1l, which is useful when planning comparative work in murine models.
The gene has gone by several names. The earlier HGNC name, DOT1-like, histone H3 methyltransferase (S. cerevisiae), refers to the yeast Dot1 protein that gave the family its name. Aliases and previous symbols include KMT4, DOT1 and KIAA1814, and UniProt lists the protein as histone-lysine N-methyltransferase, H3 lysine-79 specific. Researchers studying chromatin regulation, H3K79 methylation or the DOT1L protein will find these names in the literature and in database records.
DOT1L methylates histone H3 at Lys-79 and prefers nucleosomal substrates, making it a central enzyme for studying histone lysine methylation.
H3K79 hypermethylation by DOT1L, together with MLLT3/AF9, is linked to repression of SCNN1A at its promoter.
As a member of the class I-like SAM-binding superfamily, DOT1L is a model for nucleosome-dependent lysine methyltransferase activity.
Because DOT1L is specific for H3 Lys-79, it is a useful reference point when mapping this mark across the genome.
DOT1L is a histone methyltransferase that methylates Lys-79 of histone H3. It prefers nucleosomes to free histones and binds DNA. With MLLT3/AF9, it is involved in repressing SCNN1A transcription after H3K79 hypermethylation at the promoter.
The HGNC full name is DOT1 like histone lysine methyltransferase. Aliases and previous symbols include KMT4, DOT1 and KIAA1814. UniProt calls it histone-lysine N-methyltransferase, H3 lysine-79 specific, and an earlier HGNC name referred to the S. cerevisiae protein.
The name derives from the yeast Dot1 protein, and the mouse has an ortholog, Dot1l. DOT1L belongs to the DOT1 family of class I-like SAM-binding methyltransferases. Check the datasheet of any product for its validated reactivity.
Histone methyltransferases, transcriptional partners and target genes related to DOT1L and H3K79 methylation.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q8TEK3; product specifications from Assay Genie datasheets.