NEDD9
NEDD9, also known as HEF1, CAS-L and CASS2, is a focal adhesion scaffolding protein of the CAS family. It coordinates tyrosine-kinase-based signalling linked to cell adhesion, migration and invasion. Browse Assay Genie's current range of NEDD9 research reagents below, or read on for the biology behind the target.
NEDD9 is a scaffolding protein that plays a central coordinating role in tyrosine-kinase-based signalling related to cell adhesion. As a focal adhesion protein, it contributes to embryonic fibroblast migration. It may also be important in integrin beta-1 or B cell antigen receptor (BCR) mediated signalling in B and T cells, where integrin beta-1 stimulation leads to recruitment of proteins including CRKL and SHPTP2 to its tyrosine-phosphorylated form.
In the immune system, NEDD9 promotes adhesion and migration of lymphocytes and is required for their correct migration to the spleen and other secondary lymphoid organs. It also contributes to organisation of the T-cell F-actin cortical cytoskeleton and to centralisation of T-cell receptor microclusters at the immunological synapse. Beyond immune cells, NEDD9 negatively regulates cilia outgrowth in polarised cysts and modulates cilia disassembly by activating AURKA-mediated phosphorylation of HDAC6, followed by deacetylation of alpha-tubulin. It positively regulates RANKL-induced osteoclastogenesis and is required for maintaining hippocampal dendritic spines in the dentate gyrus and CA1 regions, linking it to spatial learning and memory.
The NEDD9 gene belongs to the CRK-associated substrates (CAS) family, whose members are adhesion docking molecules mediating protein-protein interactions in signal transduction. NCBI describes NEDD9 as a regulator of signalling complexes important in cell attachment, migration, invasion, apoptosis and the cell cycle, and it has been reported to have a role in cancer metastasis. Alternative splicing produces multiple transcript variants. The protein is also known as enhancer of filamentation 1, and the mouse ortholog is Nedd9. Its reported locations include the cell cortex, lamellipodium, focal adhesions, spindle pole, cilium basal body and nucleus.
NEDD9 is a focal adhesion scaffold that regulates signalling complexes governing cell attachment, migration and invasion, including embryonic fibroblast migration.
NEDD9 supports lymphocyte adhesion and migration to secondary lymphoid organs and organises the T-cell cytoskeleton at the immunological synapse.
NEDD9 has been reported to play a role in cancer metastasis, reflecting its control of invasion, apoptosis and the cell cycle.
NEDD9 regulates cilia disassembly through AURKA and HDAC6 and maintains hippocampal dendritic spines needed for spatial learning and memory.
NEDD9 is a scaffolding protein that coordinates tyrosine-kinase-based signalling related to cell adhesion. It promotes cell and lymphocyte migration, helps organise the T-cell cytoskeleton, regulates cilia disassembly, supports RANKL-induced osteoclastogenesis and maintains hippocampal dendritic spines.
NEDD9 is also called HEF1, CAS-L and CASS2, and its UniProt protein name is enhancer of filamentation 1. The HGNC full name is neural precursor cell expressed, developmentally down-regulated 9. Alternative splicing gives multiple transcript variants.
NCBI reports a role for NEDD9 in cancer metastasis. The gene has a mouse ortholog, Nedd9, and the protein belongs to the CAS family. Check the datasheet of any product for its validated reactivity.
Other NEDD-named proteins with pages at Assay Genie; note that these are distinct genes from the NEDD9 scaffold protein.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q14511; product specifications from Assay Genie datasheets.