MYOM2
MYOM2 (myomesin 2) is a major component of the vertebrate myofibrillar M band, where it binds myosin, titin and light meromyosin. The protein was previously named myomesin (M-protein) 2 (165kD) and is encoded by the MYOM2 gene. Browse Assay Genie's current range of MYOM2 research reagents below, or read on for the biology behind the target.
MYOM2 is a major component of the vertebrate myofibrillar M band. It binds myosin, titin and light meromyosin, and this binding is dose dependent. In the sarcomere it localises to the M line, the central structure that links thick filaments together. The giant protein titin, together with its associated proteins, interconnects the major structures of sarcomeres, the M bands and Z discs. The C-terminal end of titin extends into the M line, where it binds tightly to M-band constituents of apparent molecular masses of 190 kD and 165 kD.
The predicted MYOM2 protein contains 1,465 amino acids. Like MYOM1, it has a unique N-terminal domain followed by 12 repeat domains with strong homology to either fibronectin type III or immunoglobulin C2 domains. HGNC places the MYOM2 gene in the I-set domain containing group and the myosin binding proteins group. It is also listed among the microRNA protein coding host genes.
Protein sequence comparisons suggested that the MYOM2 protein and bovine M protein are identical, which explains the earlier designation myomesin (M-protein) 2 (165kD). Researchers studying M-band architecture often examine MYOM2 together with the related proteins MYOM1 and MYOM3, and with its binding partner titin.
MYOM2 is a major M-band component, making it relevant to studies of how thick filaments are organised and anchored within the sarcomere.
The C-terminal end of titin binds tightly to the 165 kD M-band constituent, so MYOM2 is useful for examining titin-based sarcomere linkage.
MYOM2 binds myosin and light meromyosin in a dose-dependent manner, supporting research into myosin-associated scaffolding in myofibrils.
MYOM2 shares its domain layout with MYOM1, with fibronectin type III and immunoglobulin C2 repeats, allowing comparison across myomesin proteins.
MYOM2 is a major component of the vertebrate myofibrillar M band. It binds myosin, titin and light meromyosin, and the binding is dose dependent. Through titin, whose C-terminal end extends into the M line, it helps interconnect sarcomere structures.
The HGNC full name is myomesin 2, and UniProt lists the protein as Myomesin-2. Earlier names were myomesin (M-protein) 2 (165kD) and myomesin (M-protein) 2, 165kDa. No alternative gene symbols are recorded. The protein appears to be identical to bovine M protein.
Like MYOM1, MYOM2 has a unique N-terminal domain followed by 12 repeat domains homologous to fibronectin type III or immunoglobulin C2 domains. Both are M-band proteins, and MYOM3 is a further related myomesin. Check the datasheet of any product for its validated reactivity.
Other myomesin family members and sarcomere proteins with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt P54296; product specifications from Assay Genie datasheets.