ZNRF2
ZNRF2 (zinc and ring finger 2, also known as RNF202) is a RING finger E3 ubiquitin-protein ligase linked to neuronal transmission and plasticity. It ubiquitinates the Na(+)/K(+) ATPase alpha-1 subunit and acts as a positive regulator of mTORC1 activation by amino acids. Browse Assay Genie's current range of ZNRF2 research reagents below, or read on for the biology behind the target.
ZNRF2 is an E3 ubiquitin-protein ligase that plays a role in the establishment and maintenance of neuronal transmission and plasticity. One of its characterised substrates is the Na(+)/K(+) ATPase alpha-1 subunit, ATP1A1. By ubiquitinating ATP1A1, ZNRF2 influences its endocytosis and/or degradation.
ZNRF2 also acts as a positive regulator of mTORC1 activation by amino acids, functioning upstream of the V-ATPase and of the Rag-GTPases. The relationship is self-limiting: phosphorylation by mTOR inhibits ZNRF2 by targeting it to the cytosol, which creates a feedback mechanism. NCBI Gene additionally describes the protein as involved in positive regulation of autophagosome-lysosome fusion, and as part of a protein-containing complex.
UniProt places the ZNRF2 protein at the endosome membrane, lysosome membrane, presynaptic cell membrane and in the cytoplasm, while NCBI Gene lists it at the cytoplasmic vesicle membrane and plasma membrane. The ZNRF2 gene is classed by HGNC among the ring finger proteins, the RING finger E3 ubiquitin protein ligases and the microRNA protein coding host genes. Its previous name was zinc and ring finger 2, E3 ubiquitin protein ligase, and RNF202 is a previous symbol. The mouse ortholog is Znrf2.
ZNRF2 contributes to establishing and maintaining neuronal transmission and plasticity, and it is found at the presynaptic cell membrane.
ZNRF2 positively regulates mTORC1 activation by amino acids upstream of the V-ATPase and Rag-GTPases, and is itself inhibited by mTOR phosphorylation.
Ubiquitination of the Na(+)/K(+) ATPase alpha-1 subunit by ZNRF2 influences its endocytosis and degradation.
ZNRF2 is involved in positive regulation of autophagosome-lysosome fusion and localises to endosomal and lysosomal membranes.
ZNRF2 is an E3 ubiquitin-protein ligase that supports neuronal transmission and plasticity. It ubiquitinates ATP1A1, affecting its endocytosis and/or degradation, and positively regulates mTORC1 activation by amino acids. NCBI Gene also links it to autophagosome-lysosome fusion.
The HGNC name is zinc and ring finger 2, and UniProt calls the protein E3 ubiquitin-protein ligase ZNRF2. RNF202 is an alternative symbol. The previous gene name was zinc and ring finger 2, E3 ubiquitin protein ligase. The enzyme is classified as EC 2.3.2.27.
A mouse ortholog, Znrf2, is recorded for the human gene. No rat ortholog is given in the data used here. Researchers working in other species should check the datasheet of any product for its validated reactivity.
Other E3 ubiquitin ligases, mTORC1 pathway components and Na+/K+ ATPase subunits with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q8NHG8; product specifications from Assay Genie datasheets.