SIRT2
SIRT2 (sirtuin 2, also known as SIR2L) is a human member of the sirtuin family, named as a homologue of the yeast Sir2 protein. It is catalogued as NAD-dependent protein deacetylase sirtuin-2 and belongs to class I of the sirtuins. Browse Assay Genie's current range of SIRT2 research reagents below, or read on for the biology behind the target.
SIRT2 is a member of the sirtuin family, whose members are characterised by a sirtuin core domain and are grouped into four classes. SIRT2 sits in class I. The protein is listed as an NAD-dependent protein deacetylase, and the NCBI Gene summary notes that human sirtuins may also act as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The same summary states that the functions of human sirtuins have not yet been fully determined, so SIRT2 is best approached as a protein whose activities are still being defined. A UniProt annotation also records that the protein lacks deacetylation activity, at least toward known SIRT2 targets, which is worth bearing in mind when designing activity-focused experiments.
The SIRT2 gene encodes a protein that is homologous to yeast Sir2. In yeast, sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA, and this provides the conceptual background for work on the human family. UniProt lists a broad subcellular distribution for SIRT2: the nucleus, the cytoplasm including the perinuclear region, and cytoskeletal structures such as the centrosome, centriole and spindle. It is also annotated at the midbody, on chromosomes, in the perikaryon, in cell projections including the growth cone, and at the myelin membrane. Alternative splicing of the gene gives rise to several transcript variants.
The gene symbol SIRT2 is approved by HGNC under the gene group Sirtuins, with SIR2L as an alias. Earlier names described it as a silent mating type information regulation 2 homologue from S. cerevisiae, reflecting its yeast origins. Orthologous Sirt2 genes are present in mouse and rat, which allows comparative studies in common model organisms. Other human sirtuins, from SIRT1 to SIRT7, share the family core domain and are often studied alongside SIRT2 to place its behaviour in context.
SIRT2 is a class I sirtuin with a conserved core domain, making it a reference point for comparing the roles of the human sirtuin family.
Yeast Sir2 homologues regulate epigenetic gene silencing and suppress rDNA recombination, motivating investigation of SIRT2 in related regulatory processes.
SIRT2 is annotated at the centrosome, centriole, spindle and midbody, linking it to structures involved in cell division.
Reported deacetylase and proposed mono-ADP-ribosyltransferase activities, together with a noted lack of activity toward known targets, make SIRT2 catalytic function an open question.
SIRT2 is an NAD-dependent protein deacetylase of the class I sirtuin family. NCBI notes that the functions of human sirtuins are not yet fully determined, and that they may act as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. UniProt also notes a lack of deacetylation activity toward known SIRT2 targets.
SIRT2 is also known as SIR2L, and its full name is sirtuin 2. Older names refer to it as a silent mating type information regulation 2 homologue from S. cerevisiae. Alternative splicing of the gene produces several transcript variants.
SIRT2 is a homologue of the yeast Sir2 protein, and orthologous Sirt2 genes exist in mouse and rat. Yeast sirtuins regulate epigenetic gene silencing and suppress rDNA recombination. Check the datasheet of any product for its validated reactivity.
Other members of the human sirtuin family, which share the sirtuin core domain with SIRT2.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q8IXJ6; product specifications from Assay Genie datasheets.