SynonymsSIR2L,SIR2-L,SIR2L2,Silent Information Regulator 2 Protein,NAD-Dependent Deacetylase Sirtuin-2,Silent Mating Type Information Regulation 2 Homolog
ReactivityHuman
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
SKU:
HUDL02688
UPC:
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q8IXJ6
Sensitivity:
0.61ng/mL
Range:
1.56-100ng/mL
ELISA Type:
Sandwich
Synonyms:
SIR2L,SIR2-L,SIR2L2,Silent Information Regulator 2 Protein,NAD-Dependent Deacetylase Sirtuin-2,Silent Mating Type Information Regulation 2 Homolog
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
The Human SIRT2 (Sirtuin 2) ELISA Kit is a reliable and accurate tool for measuring levels of Sirtuin 2 in human samples, including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers reproducible results, making it suitable for a variety of research applications.Sirtuin 2 is a key protein involved in regulating cellular metabolism, stress response, and longevity.
Dysregulation of Sirtuin 2 has been linked to various diseases, including cancer, metabolic disorders, and neurodegenerative diseases. By accurately measuring Sirtuin 2 levels, researchers can gain valuable insights into these conditions and potentially identify new therapeutic targets.
Product Name:
Human Sirtuin 2 (SIRT2) ELISA Kit
Product Code:
HUDL02688
Size:
96 Assays
Target:
SIRT2
Synonyms:
SIR2L, SIR2-L, SIR2L2, Silent Information Regulator 2 Protein, NAD-Dependent Deacetylase Sirtuin-2, Silent Mating Type Information Regulation 2 Homolog
Detection Method:
Sandwich
Range:
1.56-100ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
