Human SIRT2 (NAD-dependent protein deacetylase sirtuin-2) ELISA Kit
The Human SIRT2 (NAD-dependent protein deacetylase sirtuin 2) ELISA Kit is an advanced tool for precise measurement of SIRT2 levels in various biological samples such as human serum, plasma, and cell culture supernatants. This ELISA kit is known for its outstanding sensitivity and specificity, allowing for accurate and reproducible results that are essential for a wide range of scientific research applications. SIRT2 belongs to the sirtuin family of proteins and is involved in the regulation of various cellular processes, including metabolism, gene expression, and DNA repair.
Dysregulation of SIRT2 has been linked to conditions such as cancer, neurodegenerative diseases, and metabolic disorders, making it a valuable biomarker for studying these diseases and exploring potential therapeutic interventions. With the Human SIRT2 ELISA Kit, researchers can gain insights into the role of SIRT2 in health and disease, ultimately leading to a better understanding of its molecular mechanisms and potential clinical applications. Invest in this cutting-edge ELISA kit to advance your research in the field of sirtuin biology.
Product Name:
Human SIRT2 (NAD-dependent protein deacetylase sirtuin-2) ELISA Kit
SKU:
HUFI06513
Reactivity:
Human
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
9.375 pg/mL
Range:
15.625-1000 pg/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
87-105%
94-104%
89-104%
EDTA Plasma (n = 5)
86-100%
82-100%
82-98%
Heparin Plasma (n = 5)
83-99%
91-100%
83-91%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
88-104
95
EDTA Plasma (n = 5)
93-105
98
Heparin Plasma (n = 5)
87-99
95
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
