CSTF2
CSTF2, also known as CstF-64, is a nuclear RNA-binding subunit of the cleavage stimulation factor complex. It contributes to polyadenylation and 3'-end cleavage of mammalian pre-mRNAs by binding directly to the pre-mRNA. Browse Assay Genie's current range of CSTF2 research reagents below, or read on for the biology behind the target.
CSTF2 (cleavage stimulation factor subunit 2) is one of the multiple factors required for polyadenylation and 3'-end cleavage of mammalian pre-mRNAs. Within the cleavage stimulation factor (CSTF) complex, this subunit is directly involved in binding to the pre-mRNA. Specifically, it binds GU-rich elements within the 3'-untranslated region of mRNAs, helping to define where the transcript is processed.
The CSTF2 gene encodes a nuclear protein that carries an RRM (RNA recognition motif) domain, the module through which it engages RNA. HGNC classifies it within the RNA binding motif containing group and the cleavage stimulation factor subunits group. The mouse ortholog is Cstf2.
The protein is widely referred to as CstF-64. Earlier gene names described it as cleavage stimulation factor, 3' pre-RNA, subunit 2, 64kD, reflecting the apparent molecular size that gave CstF-64 its name. Because it links the CSTF complex to the RNA substrate, CSTF2 is a common focus for studies of mRNA 3' end formation, and the CSTF2 protein is studied wherever polyadenylation is examined.
CSTF2 is one of the factors required for polyadenylation of mammalian pre-mRNAs, making it a core component for studying 3' end processing.
As a subunit of the CSTF complex, CSTF2 takes part in 3'-end cleavage of pre-mRNAs, supporting work on how cleavage is directed.
CSTF2 binds pre-mRNA directly through its RRM domain and recognises GU-rich elements, a useful model for RNA recognition studies.
Binding of GU-rich elements in the 3'-untranslated region links CSTF2 to research on how 3' UTR sequences shape mRNA processing.
CSTF2 is one of the factors required for polyadenylation and 3'-end cleavage of mammalian pre-mRNAs. This subunit of the cleavage stimulation factor complex is directly involved in binding to pre-mRNAs, and it recognises GU-rich elements within the 3'-untranslated region.
CSTF2 is commonly called CstF-64. Previous names include cleavage stimulation factor, 3' pre-RNA, subunit 2, 64kD, and cleavage stimulation factor, 3' pre-RNA, subunit 2. HGNC places it in the RNA binding motif containing and CSTF subunit gene groups.
A mouse ortholog, Cstf2, is recorded for the human gene. The source data do not list a rat ortholog. Researchers should check the datasheet of any product for its validated reactivity before choosing a reagent for a given species.
Other cleavage stimulation factor subunits, polyadenylation factors and RNA-binding proteins with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt P33240; product specifications from Assay Genie datasheets.