RNF7
RNF7 (ring finger protein 7, also known as RING-box protein 2, SAG, ROC2, CKBBP1 and RBX2) is a highly conserved RING-box family protein. It is an essential subunit of SKP1-cullin-F box ubiquitin ligases, which form part of the cell's protein degradation machinery. Browse Assay Genie's current range of RNF7 research reagents below, or read on for the biology behind the target.
RNF7 is a member of the RING-box family and belongs to the HGNC groups of ring finger proteins, RING finger E3 ubiquitin protein ligases and degenerate RING E3 ubiquitin ligases. It is an essential subunit of SKP1-cullin/CDC53-F box protein ubiquitin ligases, which contribute to the protein degradation machinery important for cell cycle progression and signal transduction. UniProt records it as a catalytic component of cullin-RING E3 ubiquitin-protein ligase complexes, with the enzyme activities listed under EC 2.3.2.27 and EC 2.3.2.32.
The RNF7 protein is found in the cytoplasm and the nucleus. It interacts with, and is a substrate of, casein kinase II (CSNK2A1/CKII). Phosphorylation of RNF7 by CSNK2A1 has been shown to promote the degradation of IkappaBalpha (CHUK/IKK-alpha/IKBKA) and p27Kip1 (CDKN1B). The RNF7 gene is highly conserved, with a mouse ortholog named Rnf7, and alternatively spliced transcript variants encoding distinct isoforms have been reported.
RNF7 is also known by the aliases SAG, ROC2, CKBBP1 and RBX2. In a microbial infection context, UniProt notes that after infection by HIV-1, RNF7 acts as the catalytic component of a cullin-5-RING E3 ubiquitin-protein ligase complex (ECS complex) hijacked by the HIV-1 Vif protein, which catalyses ubiquitination and degradation of APOBEC3F and APOBEC3G.
RNF7 is a catalytic RING-box subunit of cullin-RING E3 ubiquitin ligases, making it a focus for studies of ubiquitination machinery.
As an essential subunit of SKP1-cullin-F box ligases, RNF7 supports the protein degradation machinery important for cell cycle progression.
CSNK2A1 phosphorylation of RNF7 promotes degradation of IkappaBalpha and p27Kip1, linking the protein to signalling control.
RNF7 is part of the cullin-5 ECS complex hijacked by HIV-1 Vif to degrade APOBEC3F and APOBEC3G.
RNF7 is an essential subunit of SKP1-cullin/CDC53-F box protein ubiquitin ligases, which are part of the protein degradation machinery important for cell cycle progression and signal transduction. UniProt describes it as a catalytic component of cullin-RING E3 ubiquitin ligase complexes.
RNF7 (ring finger protein 7) is also called RING-box protein 2, SAG, ROC2, CKBBP1 and RBX2. NCBI Gene reports alternatively spliced transcript variants encoding distinct isoforms, so literature and databases may refer to the protein under several of these names.
NCBI Gene describes RNF7 as a highly conserved ring finger protein, with a mouse ortholog named Rnf7. It is a substrate of casein kinase II (CSNK2A1), and this phosphorylation promotes degradation of IkappaBalpha and p27Kip1. Check the datasheet of any product for its validated reactivity.
Other RING finger proteins and E3 ubiquitin ligase family members with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q9UBF6; product specifications from Assay Genie datasheets.