KIFAP3
KIFAP3 (kinesin associated protein 3, also known as SMAP, KAP3, FLA3 and KAP-1) is an armadillo-repeat protein that binds the KIF3A/KIF3B kinesin heterodimer. It helps tether chromosomes to the spindle pole and is linked to chromosome movement. Browse Assay Genie's current range of KIFAP3 research reagents below, or read on for the biology behind the target.
KIFAP3 is involved in tethering chromosomes to the spindle pole and in chromosome movement. It binds the tail domain of the KIF3A/KIF3B heterodimer to form a heterotrimeric KIF3 complex, and it may regulate the membrane binding of that complex. NCBI Gene describes it as a likely linker between the human chromosome-associated polypeptide (HCAP) and KIF3A/B, a kinesin superfamily protein in the nucleus, and as playing a role in the interaction of chromosomes with an ATPase motor protein.
The KIFAP3 gene encodes a protein with nine Armadillo repeats, and HGNC places it in the armadillo like helical domain containing group. Through these repeats it interacts with the small G protein GDP dissociation stimulator (smg GDS). This regulator has two activities on a group of small G proteins, including the Rho and Rap1 families and Ki-Ras. It stimulates their GDP/GTP exchange and inhibits their interactions with membranes. The KIFAP3 protein is highly concentrated around the endoplasmic reticulum. It is phosphorylated by v-src, and this phosphorylation reduces its affinity for smg GDS. Several transcript variants encoding different isoforms have been reported for the gene.
The protein goes by several names in the literature, including SMAP, KAP3, FLA3 and KAP-1, and its previous name was kinesin-associated protein 3. These names reflect its dual connections to small G protein regulation and to kinesin motor function. A mouse ortholog, Kifap3, is recognised. Researchers studying KIFAP3 typically focus on kinesin-2 complex assembly, mitotic chromosome behaviour and the control of membrane association by motor complexes.
KIFAP3 is involved in tethering chromosomes to the spindle pole and in chromosome movement, making it relevant to mitotic mechanics.
It binds the tail domain of the KIF3A/KIF3B heterodimer to form a heterotrimeric KIF3 complex.
Its Armadillo repeats interact with smg GDS, a regulator of Rho and Rap1 family members and Ki-Ras.
KIFAP3 may regulate the membrane binding of the KIF3 complex and is concentrated around the endoplasmic reticulum.
KIFAP3 is involved in tethering chromosomes to the spindle pole and in chromosome movement. It binds the tail domain of the KIF3A/KIF3B heterodimer to form a heterotrimeric KIF3 complex, and it may regulate the membrane binding of this complex.
KIFAP3 is also called SMAP, KAP3, FLA3 and KAP-1, and its full name is kinesin associated protein 3. NCBI Gene reports several transcript variants that encode different isoforms, so check the datasheet of any product for its validated reactivity and target region.
Its nine Armadillo repeats interact with smg GDS, a regulator of Rho and Rap1 family members and Ki-Ras. Phosphorylation of KIFAP3 by v-src reduces its affinity for smg GDS. The mouse ortholog is Kifap3.
Kinesin-2 subunits, small G proteins and other proteins linked to chromosome movement and motor-complex regulation.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q92845; product specifications from Assay Genie datasheets.