CENPF
CENPF (centromere protein F, also known as hcp-1 and formerly centromere protein F, 350/400kDa, or mitosin) is a large protein that associates with the centromere-kinetochore complex. It is required for kinetochore function and chromosome segregation in mitosis. Browse Assay Genie's current range of CENPF research reagents below, or read on for the biology behind the target.
CENPF is required for kinetochore function and chromosome segregation in mitosis. It is needed for the kinetochore localisation of dynein, LIS1, NDE1 and NDEL1. Beyond mitosis, it regulates recycling of the plasma membrane by acting as a link between recycling vesicles and the microtubule network, through its association with STX4 and SNAP25. Via its C-terminus it is also involved in regulating DNA synthesis and hence cell cycle progression.
The CENPF gene encodes a protein that belongs to the centromere protein F family. Its localisation changes through the cell cycle. It is a component of the nuclear matrix during the G2 phase of interphase. In late G2 it associates with the kinetochore and stays there until early anaphase. It then localises to the spindle midzone in late anaphase and to the intracellular bridge in telophase, and is thought to be degraded afterwards. UniProt also records it in the perinuclear cytoplasm. The protein is thought to form either a homodimer or a heterodimer.
CENPF has been linked to regulation of RB proteins during cell division and proliferation, and is described as a potential inhibitor of pocket protein-mediated processes during development. Interaction with RB directs embryonic stem cells toward a cardiac lineage, and the protein may play a role in the embryonic cardiomyocyte cell cycle, in skeletal myogenesis and in cell differentiation in embryogenesis. It is also involved in dendritic cell regulation of T-cell immunity against chlamydia. Autoantibodies against CENPF have been found in patients with cancer or graft versus host disease. The older name, mitosin, reflects its mitotic role.
CENPF is required for kinetochore function and chromosome segregation, making it a useful marker for studying mitotic progression.
CENPF regulates RB protein activity and DNA synthesis, linking it to cell cycle progression and proliferation control.
CENPF may regulate the cardiomyocyte cell cycle and skeletal myogenesis, and interaction with RB directs embryonic stem cells toward a cardiac lineage.
Autoantibodies against CENPF occur in patients with cancer or graft versus host disease, making it relevant to autoantigen research.
CENPF is required for kinetochore function and chromosome segregation in mitosis, including kinetochore localisation of dynein, LIS1, NDE1 and NDEL1. It also links recycling vesicles to the microtubule network and helps regulate cell cycle progression through RB proteins and DNA synthesis.
CENPF stands for centromere protein F. It is also known as hcp-1, and earlier names were centromere protein F, 350/400kDa (mitosin) and centromere protein F, 350/400kDa. The name mitosin reflects its role at the kinetochore during mitosis.
Autoantibodies against CENPF have been found in patients with cancer or graft versus host disease. UniProt also notes that it may promote continued mitosis in transformed, abnormally dividing neonatal cardiomyocytes. Check the datasheet of any product for its validated reactivity.
Other kinetochore, mitotic and cell cycle regulators with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt P49454; product specifications from Assay Genie datasheets.