EEF1A2
EEF1A2 (eukaryotic translation elongation factor 1 alpha 2, also known as EEF1AL, HS1, STNL or STN) is a translational GTPase that delivers aminoacyl-tRNA to the ribosome during protein synthesis. It is the alpha 2 isoform of the alpha subunit of the elongation factor-1 complex. Browse Assay Genie's current range of EEF1A2 research reagents below, or read on for the biology behind the target.
EEF1A2 is a translation elongation factor that catalyses the GTP-dependent binding of aminoacyl-tRNA (aa-tRNA) to the A-site of ribosomes during the elongation phase of protein synthesis. Base pairing between the mRNA codon and the aa-tRNA anticodon promotes GTP hydrolysis, which releases the aa-tRNA from the factor and allows it to be accommodated into the ribosome. The growing protein chain is then transferred from the P-site peptidyl tRNA to the A-site aa-tRNA, extending it by one amino acid through ribosome-catalysed peptide bond formation.
The EEF1A2 gene encodes the alpha 2 isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. The EEF1A2 protein belongs to the TRAFAC class translation factor GTPase superfamily, within the classic translation factor GTPase family and the EF-Tu/EF-1A subfamily. HGNC places it in the translational GTPases gene group, and UniProt records it at the endoplasmic reticulum membrane. Its tissue expression is distinct from that of the alpha 1 isoform (EEF1A1): EEF1A2 is expressed in brain, heart and skeletal muscle, whereas EEF1A1 is expressed in brain, placenta, lung, liver, kidney and pancreas.
The gene has previously been known as statin-like and statin, which accounts for the aliases STN and STNL, alongside EEF1AL and HS1. NCBI Gene notes that the EEF1A2 gene may be critical in the development of ovarian cancer. Mouse and rat orthologs are both named Eef1a2, which allows the factor to be studied in common model systems.
EEF1A2 delivers aminoacyl-tRNA to the ribosomal A-site, making it a core component for studying the elongation phase of translation.
As a member of the EF-Tu/EF-1A subfamily, EEF1A2 illustrates codon-driven GTP hydrolysis and aa-tRNA release at the ribosome.
EEF1A2 is expressed in brain, heart and skeletal muscle, which makes it relevant to tissue-specific control of translation.
NCBI Gene reports that EEF1A2 may be critical in the development of ovarian cancer, prompting investigation of its role in tumour biology.
EEF1A2 is a translation elongation factor that catalyses GTP-dependent binding of aminoacyl-tRNA to the ribosomal A-site. Codon–anticodon pairing triggers GTP hydrolysis and aa-tRNA release, so the ribosome can form the next peptide bond and extend the growing chain by one amino acid.
The HGNC name is eukaryotic translation elongation factor 1 alpha 2, and UniProt uses elongation factor 1-alpha 2. Aliases include EEF1AL, HS1, STNL and STN; the last two come from the earlier names statin-like and statin. The gene encodes the alpha 2 isoform.
Both are alpha isoforms of the elongation factor-1 complex. EEF1A2 is expressed in brain, heart and skeletal muscle, while EEF1A1 is found in brain, placenta, lung, liver, kidney and pancreas. Mouse and rat both have an Eef1a2 ortholog; check the datasheet of any product for its validated reactivity.
Other translation elongation factors and regulators of protein synthesis with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q05639; product specifications from Assay Genie datasheets.