NFE2
NFE2 (nuclear factor, erythroid 2, also written NF-E2) is the 45 kDa subunit of the NF-E2 transcription factor complex. It is a bZIP protein of the CNC subfamily that regulates erythroid and megakaryocytic maturation and differentiation. Browse Assay Genie's current range of NFE2 research reagents below, or read on for the biology behind the target.
NFE2 is a component of the NF-E2 complex, which is essential for regulating erythroid and megakaryocytic maturation and differentiation. The NFE2 subunit recognises the TCAT/C sequence of the AP-1-like core palindrome found in a number of erythroid and megakaryocytic gene promoters. It also binds the hypersensitive site 2 (HS2) of the beta-globin locus control region. To bind the NF-E2 motif, NFE2 requires MAFK or other small MAF proteins. It may play a role in all aspects of haemoglobin production, from globin and haem synthesis to the procurement of iron.
The NFE2 protein belongs to the bZIP family and the CNC subfamily, and HGNC places the NFE2 gene in the NFE2 family of bZIP transcription factors. UniProt lists its subcellular location as the nucleus, specifically PML bodies, and the cytoplasm. NCBI Gene annotates NFE2 with WW domain binding, identical protein binding and sequence-specific double-stranded DNA binding activities. It also contributes to cis-regulatory region sequence-specific DNA binding and forms part of a protein-DNA complex. It is predicted to be involved in regulating transcription by RNA polymerase II.
Predicted biological processes linked to NFE2 include labyrinthine layer blood vessel development, negative regulation of bone mineralisation and positive regulation of peptidyl-lysine acetylation. The protein was formerly named nuclear factor (erythroid-derived 2), 45kD, and NF-E2 remains a common alias. Mouse and rat both carry an orthologous Nfe2 gene, which allows comparative work in rodent systems.
NFE2 is part of the NF-E2 complex that regulates erythroid maturation and differentiation, making it a focus for studies of red cell development.
The NF-E2 complex is essential for megakaryocytic maturation and differentiation, so NFE2 is relevant to work on megakaryocyte gene regulation.
NFE2 binds hypersensitive site 2 of the beta-globin control region, linking it to transcriptional control of the globin locus.
NFE2 may act across haemoglobin production, from globin and haem synthesis to iron procurement, supporting studies of these coordinated pathways.
NFE2 is a component of the NF-E2 complex, which regulates erythroid and megakaryocytic maturation and differentiation. It recognises the TCAT/C AP-1-like motif in erythroid and megakaryocytic promoters and binds the beta-globin control region HS2. It needs MAFK or other small MAF proteins to bind DNA.
NFE2 is also written NF-E2, and its UniProt name is transcription factor NF-E2 45 kDa subunit. HGNC records the previous names nuclear factor (erythroid-derived 2), 45kD, and 45kDa. It sits in the NFE2 family of bZIP transcription factors, alongside NFE2L1 and NFE2L3.
Yes. Mouse and rat each have an orthologous gene named Nfe2. This allows the NF-E2 pathway to be studied in rodent models, though you should check the datasheet of any product for its validated reactivity.
Other NFE2 family bZIP transcription factors with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q16621; product specifications from Assay Genie datasheets.