BHLHA15
BHLHA15, also known as MIST1 or BHLHB8, is a nuclear class A basic helix-loop-helix transcription factor. It binds E-box DNA sequences and regulates acinar cell identity, exocrine cell organisation and the restraint of myoblast differentiation. Browse Assay Genie's current range of BHLHA15 research reagents below, or read on for the biology behind the target.
BHLHA15 is a sequence-specific DNA-binding transcription factor that recognises the E-box consensus sequence 5'-CANNTG-3'. It helps control the transcriptional activity of MYOD1, keeping expanding myoblast populations undifferentiated. This repression may occur through occupancy of muscle-specific E-boxes by BHLHA15 homodimers. An N-terminal repressor domain may also allow it to negatively regulate bHLH-mediated transcription more broadly.
Beyond muscle, BHLHA15 is a key regulator of acinar cell function, stability and identity. It is also required for normal organelle localisation in exocrine cells and for mitochondrial calcium ion transport. UniProt describes it as a possible regulator of gene expression in several embryonic and postnatal cell lineages. The protein is located in the nucleus, and NCBI Gene predicts an association with chromatin.
NCBI Gene lists predicted roles for the BHLHA15 gene in the cellular response to glucose starvation, the endoplasmic reticulum unfolded protein response, negative regulation of myotube differentiation, cadherin-mediated cell-cell adhesion and the intracellular distribution of mitochondria. The protein belongs to the HGNC group of basic helix-loop-helix proteins. Its previous symbol, BHLHB8, derives from an earlier class B naming scheme, and MIST1 remains a widely used alias. Mouse and rat both carry a Bhlha15 ortholog.
BHLHA15 is a key regulator of acinar cell function, stability and identity, making it central to studies of exocrine cell maintenance.
By controlling MYOD1 activity, BHLHA15 helps keep expanding myoblast populations undifferentiated, so it is relevant to muscle lineage research.
BHLHA15 is required for mitochondrial calcium ion transport and normal organelle localisation in exocrine cells.
As an E-box-binding bHLH factor with a repressor domain, BHLHA15 is a model for studying gene control across cell lineages.
BHLHA15 is a nuclear transcription factor that binds the E-box sequence 5'-CANNTG-3'. It restrains MYOD1 activity so myoblasts stay undifferentiated, and it regulates acinar cell function, stability and identity. It is also needed for organelle localisation in exocrine cells and mitochondrial calcium transport.
Yes. MIST1 is an alias of BHLHA15. The previous symbol BHLHB8 reflects the earlier name 'basic helix-loop-helix domain containing, class B, 8'. The current HGNC name is basic helix-loop-helix family member a15, and UniProt calls it class A basic helix-loop-helix protein 15.
Yes. Both mouse and rat have an ortholog named Bhlha15, so findings on the protein can be studied in common rodent models. Check the datasheet of any product for its validated reactivity.
Other basic helix-loop-helix transcription factors, myogenic regulators and related nuclear proteins with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q7RTS1; product specifications from Assay Genie datasheets.