NCF2
NCF2 (neutrophil cytosolic factor 2, also known as p67phox or NOXA2) is the 67-kilodalton cytosolic subunit of the phagocyte NADPH oxidase complex. It contributes to the production of superoxide in neutrophils and is a member of the NCF2/NOXA1 family. Browse Assay Genie's current range of NCF2 research reagents below, or read on for the biology behind the target.
NCF2 is a subunit of the phagocyte NADPH oxidase complex, which transfers electrons from cytosolic NADPH to molecular oxygen to produce the superoxide anion. In the activated complex, electrons pass from NADPH to flavin adenine dinucleotide (FAD) and then via two heme molecules to oxygen, generating superoxide through an outer-sphere reaction. In neutrophils this oxidase produces a burst of superoxide that is delivered to the lumen of the phagosome.
NCF2 is held in the cytoplasm until the oxidase is activated. Activation begins when the cytosolic subunits assemble with the core NADPH oxidase complex at the plasma membrane or the phagosomal membrane. This is initiated by phosphorylation-dependent binding of the cytosolic subunit NCF1 (p47phox) to the C-terminus of CYBA (p22phox). The NCF2 gene encodes the 67-kilodalton cytosolic subunit, and alternative splicing results in multiple transcript variants that encode different isoforms. The HGNC places the protein in the tetratricopeptide repeat domain containing and SH3 domain containing gene groups.
The gene was originally named neutrophil cytosolic factor 2 (65kD, chronic granulomatous disease, autosomal 2), which reflects its disease association. Mutations in NCF2, as in other NADPH oxidase subunits, can result in chronic granulomatous disease, which causes recurrent infections by catalase-positive organisms. Orthologous genes are named Ncf2 in both mouse and rat.
NCF2 is a cytosolic subunit of the complex that transfers electrons from NADPH to oxygen, so it is central to studies of superoxide production.
Mutations in NCF2 and other NADPH oxidase subunits can cause chronic granulomatous disease, with recurrent infections by catalase-positive organisms.
The oxidase delivers a burst of superoxide into the neutrophil phagosome, and NCF2 is one of the subunits involved in this response.
Cytosolic subunits including NCF2 assemble with the core complex at the plasma or phagosomal membrane, making it useful for studying activation.
NCF2 is a cytosolic subunit of the phagocyte NADPH oxidase complex. When the cytosolic subunits assemble with the core complex at the plasma or phagosomal membrane, the oxidase transfers electrons from NADPH to oxygen, producing superoxide. In neutrophils this creates a superoxide burst in the phagosome.
NCF2 is also called p67phox and NOXA2, and its full name is neutrophil cytosolic factor 2. An older name was neutrophil cytosolic factor 2 (65kD, chronic granulomatous disease, autosomal 2). Alternative splicing gives multiple transcript variants that encode different isoforms.
Mutations in NCF2 can result in chronic granulomatous disease, which causes recurrent infections by catalase-positive organisms. Mouse and rat both have an orthologous Ncf2 gene. Check the datasheet of any product for its validated reactivity.
Other neutrophil cytosolic factors and NADPH oxidase subunits with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt P19878; product specifications from Assay Genie datasheets.