Anti-NOXA2/P67phox Antibody (CAB1178)

Antibody Name:	Anti-NOXA2 / P67phox Antibody
Antibody SKU:	CAB1178
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 227-526 of human NOXA2 / P67phox (NP_000424.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	THP-1, Mouse spleen

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 227-526 of human NOXA2 / P67phox (NP_000424.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	PPPR PKTP EIFR ALEG EAHR VLFG FVPE TKEE LQVM PGNI VFVL KKGN DNWA TVMF NGQK GLVP CNYL EPVE LRIH PQQQ PQEE SSPQ SDIP APPS SKAP GRPQ LSPG QKQK EEPK EVKL SVPM PYTL KVHY KYTV VMKT QPGL PYSQ VRDM VSKK LELR LEHT KLSY RPRD SNEL VPLS EDSM KDAW GQVK NYCL TLWC ENTV GDQG FPDE PKES EKAD ANNQ TTEP QLKK GSQV EALF SYEA TQPE DLEF QEGD IILV LSKV NEEW LEGE CKGK VGIF PKVF VEDC ATTD LEST RREV
Gene ID:	4688
Uniprot:	P19878
Cellular Location:	Cytoplasm
Calculated MW:	47kDa/50kDa/54kDa/59kDa
Observed MW:	68kDa

Synonyms:

NCF2, NCF-2, NOXA2, P67-PHOX, P67PHOX

Background:

This gene encodes neutrophil cytosolic factor 2, the 67-kilodalton cytosolic subunit of the multi-protein NADPH oxidase complex found in neutrophils. This oxidase produces a burst of superoxide which is delivered to the lumen of the neutrophil phagosome. Mutations in this gene, as well as in other NADPH oxidase subunits, can result in chronic granulomatous disease, a disease that causes recurrent infections by catalase-positive organisms. Alternative splicing results in multiple transcript variants encoding different isoforms.

UniProt Protein Function:	p67phox: NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production). Interacts with SYTL1 and RAC1. Interacts with NCF4. May interact with NOXO1. Belongs to the NCF2/NOXA1 family.
UniProt Protein Details:	Protein type:Oxidoreductase Chromosomal Location of Human Ortholog: 1q25 Cellular Component: cytoplasm; acrosome; nucleolus; cytosol; NADPH oxidase complex Molecular Function:protein C-terminus binding; protein binding; electron carrier activity; Rac GTPase binding Biological Process: respiratory burst; response to drug; interaction with host; superoxide metabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; response to lipopolysaccharide; cellular response to hormone stimulus; antigen processing and presentation of peptide antigen via MHC class I; response to hyperoxia; positive regulation of neuron apoptosis; response to glucose stimulus; cellular defense response; innate immune response; antigen processing and presentation of exogenous peptide antigen via MHC class I; response to activity; vascular endothelial growth factor receptor signaling pathway; response to progesterone stimulus; superoxide release; positive regulation of blood pressure; aging Disease: Granulomatous Disease, Chronic, Autosomal Recessive, Cytochrome B-positive, Type Ii
NCBI Summary:	This gene encodes neutrophil cytosolic factor 2, the 67-kilodalton cytosolic subunit of the multi-protein NADPH oxidase complex found in neutrophils. This oxidase produces a burst of superoxide which is delivered to the lumen of the neutrophil phagosome. Mutations in this gene, as well as in other NADPH oxidase subunits, can result in chronic granulomatous disease, a disease that causes recurrent infections by catalase-positive organisms. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2010]
UniProt Code:	P19878
NCBI GenInfo Identifier:	1346669
NCBI Gene ID:	4688
NCBI Accession:	P19878.2
UniProt Secondary Accession:	P19878,Q2PP06, Q8NFC7, Q9BV51, B2R6Q1, B4DKQ7, B4DQA7 E9PHJ2, E9PHX3,
UniProt Related Accession:	P19878
Molecular Weight:	50,337 Da
NCBI Full Name:	Neutrophil cytosol factor 2
NCBI Synonym Full Names:	neutrophil cytosolic factor 2
NCBI Official Symbol:	NCF2
NCBI Official Synonym Symbols:	NCF-2; NOXA2; P67PHOX; P67-PHOX
NCBI Protein Information:	neutrophil cytosol factor 2; NADPH oxidase activator 2; 67 kDa neutrophil oxidase factor; neutrophil NADPH oxidase factor 2; chronic granulomatous disease, autosomal 2; neutrophil cytosolic factor 2 (65kD, chronic granulomatous disease, autosomal 2)
UniProt Protein Name:	Neutrophil cytosol factor 2
UniProt Synonym Protein Names:	67 kDa neutrophil oxidase factor; NADPH oxidase activator 2; Neutrophil NADPH oxidase factor 2; p67-phox
UniProt Gene Name:	NCF2
UniProt Entry Name:	NCF2_HUMAN

	Western blot analysis of extracts of various cell lines, using NOXA2 / P67phox antibody (CAB1178) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunohistochemistry of paraffin-embedded human esophageal cancer using NOXA2 / P67phox Rabbit pAb (CAB1178) at dilution of 1:100 (40x lens).

Antibodies

Anti-NOXA2/p67phox Antibody (CAB3703)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)