ACAT2
ACAT2 (acetyl-CoA acetyltransferase 2) is a cytosolic thiolase involved in lipid metabolism and in the biosynthetic pathway of cholesterol. It is also known as cytosolic acetoacetyl-CoA thiolase. Browse Assay Genie's current range of ACAT2 research reagents below, or read on for the biology behind the target.
ACAT2 is an acetyl-CoA acetyltransferase (EC 2.3.1.9) that localises to the cytosol. According to UniProt, it is involved in the biosynthetic pathway of cholesterol. NCBI Gene describes the gene product as an enzyme of lipid metabolism that encodes cytosolic acetoacetyl-CoA thiolase, which is why the protein is often referred to by that descriptive name in the literature.
The ACAT2 gene belongs to the HGNC group of acetyl-CoA acetyltransferases, and the protein is a member of the thiolase family within the thiolase-like superfamily. Its closest relative among the targets listed here is ACAT1. The human gene sits at 6q25.3, and mouse and rat each have an orthologous Acat2 gene, so the enzyme can be studied in common rodent models. Alternatively spliced transcript variants encoding different isoforms have been reported for this gene.
The gene has an unusual genomic arrangement. NCBI Gene notes that it shows complementary overlapping with the 3-prime region of the TCP1 gene in both mouse and human. The two genes lie on opposite strands of DNA and are transcribed in opposite orientations. The earlier HGNC name, acetyl-Coenzyme A acetyltransferase 2 (acetoacetyl Coenzyme A thiolase), reflects the activity of the enzyme. Researchers working on sterol synthesis and lipid metabolism often study ACAT2 alongside other thiolase family members, using an ACAT2 antibody, ACAT2 ELISA kit or ACAT2 protein as appropriate to the question.
ACAT2 is involved in the biosynthetic pathway of cholesterol, making it a relevant enzyme for studies of sterol production.
NCBI Gene classes the ACAT2 product as an enzyme of lipid metabolism, encoding cytosolic acetoacetyl-CoA thiolase.
As a thiolase family member related to ACAT1, ACAT2 supports comparative work on acetyl-CoA acetyltransferases and their distinct cellular roles.
The overlap with TCP1 on opposite strands and the alternatively spliced isoforms make ACAT2 a useful subject for gene regulation studies.
ACAT2 is a cytosolic acetyl-CoA acetyltransferase (EC 2.3.1.9), also called cytosolic acetoacetyl-CoA thiolase. UniProt annotates it as involved in the biosynthetic pathway of cholesterol, and NCBI Gene describes it as an enzyme of lipid metabolism.
The HGNC name is acetyl-CoA acetyltransferase 2, and UniProt lists acetyl-CoA acetyltransferase, cytosolic. Previous names include acetyl-Coenzyme A acetyltransferase 2 (acetoacetyl Coenzyme A thiolase). No alternative gene symbols are recorded. NCBI Gene reports alternatively spliced transcript variants encoding different isoforms.
Yes. Mouse and rat each have an orthologous gene named Acat2. NCBI Gene also notes that the overlap with the 3-prime region of TCP1, on the opposite strand, is seen in both mouse and human. Check the datasheet of any product for its validated reactivity.
Other acetyl-CoA acetyltransferases and thiolase family members involved in lipid metabolism.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q9BWD1; product specifications from Assay Genie datasheets.