Anti-ACAT2 Antibody (CAB7866)

Antibody Name:	Anti-ACAT2 Antibody
Antibody SKU:	CAB7866
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-97 of human ACAT2 (NP_005882.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	BT-474, MCF7, HeLa, HepG2, PC-12, Mouse liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-97 of human ACAT2 (NP_005882.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MNAG SDPV VIVS AART IIGS FNGA LAAV PVQD LGST VIKE VLKR ATVA PEDV SEVI FGHV LAAG CGQN PVRQ ASVG AGIP YSVP AWSC QMIC GSGL K
Gene ID:	39
Uniprot:	Q9BWD1
Cellular Location:	Cytoplasm
Calculated MW:	41kDa/44kDa
Observed MW:	41kDa

Synonyms:

ACAT2

Background:

The product of this gene is an enzyme involved in lipid metabolism, and it encodes cytosolic acetoacetyl-CoA thiolase. This gene shows complementary overlapping with the 3-prime region of the TCP1 gene in both mouse and human. These genes are encoded on opposite strands of DNA, as well as in opposite transcriptional orientation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	ACAT2: The product of this gene is an enzyme involved in lipid metabolism, and it encodes cytosolic acetoacetyl-CoA thiolase. This gene shows complementary overlapping with the 3-prime region of the TCP1 gene in both mouse and human. These genes are encoded on opposite strands of DNA, as well as in opposite transcriptional orientation. [provided by RefSeq, Jul 2008]
UniProt Protein Details:	Protein type:Carbohydrate Metabolism - butanoate; Lipid Metabolism - synthesis and degradation of ketone bodies; Carbohydrate Metabolism - pyruvate; Amino Acid Metabolism - valine, leucine and isoleucine degradation; Amino Acid Metabolism - tryptophan; Acetyltransferase; Secondary Metabolites Metabolism - terpenoid backbone biosynthesis; Carbohydrate Metabolism - propanoate; EC 2.3.1.9; Lipid Metabolism - fatty acid; Amino Acid Metabolism - lysine degradation Chromosomal Location of Human Ortholog: 6q25.3 Cellular Component: nucleoplasm; cytoplasm; nucleolus; nucleus Molecular Function:acetyl-CoA C-acetyltransferase activity Biological Process: lipid metabolic process Disease: Acetyl-coa Acetyltransferase-2 Deficiency
NCBI Summary:	The product of this gene is an enzyme involved in lipid metabolism, and it encodes cytosolic acetoacetyl-CoA thiolase. This gene shows complementary overlapping with the 3-prime region of the TCP1 gene in both mouse and human. These genes are encoded on opposite strands of DNA, as well as in opposite transcriptional orientation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2014]
UniProt Code:	Q9BWD1
NCBI GenInfo Identifier:	52000838
NCBI Gene ID:	39
NCBI Accession:	Q9BWD1.2
UniProt Related Accession:	Q9BWD1
Molecular Weight:	41 kDa
NCBI Full Name:	Acetyl-CoA acetyltransferase, cytosolic
NCBI Synonym Full Names:	acetyl-CoA acetyltransferase 2
NCBI Official Symbol:	ACAT2
NCBI Protein Information:	acetyl-CoA acetyltransferase, cytosolic
UniProt Protein Name:	Acetyl-CoA acetyltransferase, cytosolic
UniProt Synonym Protein Names:	Acetyl-CoA transferase-like protein; Cytosolic acetoacetyl-CoA thiolase
Protein Family:	Acetyl-CoA acetyltransferase
UniProt Gene Name:	ACAT2
UniProt Entry Name:	THIC_HUMAN

	Western blot analysis of extracts of various cell lines, using ACAT2 antibody (CAB7866) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat ovary using ACAT2 antibody (CAB7866) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human colon carcinoma using ACAT2 antibody (CAB7866) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human oophoroma using ACAT2 antibody (CAB7866) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse kidney using ACAT2 antibody (CAB7866) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
ACAT2 Antibody (PACO07552)	Human ACAT2 / Acetyl Coenzyme A Acetyltransferase 2, cytosolic ELISA Kit
	Human ACAT2 (Acetyl Coenzyme A Acetyltransferase 2, cytosolic) CLIA Kit (HUES00124)
	Human ACAT2 (Acetyl Coenzyme A Acetyltransferase 2, cytosolic) ELISA Kit (HUES01415)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)