The MRPS25 Antibody (CAB9083) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By targeting the MRPS25 protein, this antibody enables accurate detection and analysis in various cell types, making it a valuable asset for investigations in molecular biology and mitochondrial function research.MRPS25 is a key player in mitochondrial protein synthesis and function, making it a crucial component in cellular metabolism and energy production.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
MRPS25 Antibody
SKU:
CAB9083
Size:
20μL, 100μL
Reactivity:
Human
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:
KILG KNEE TLRE EEEE KKQL SHPA NFGP RKYC LREC ICEV EGQV PCPS LVPL PKEM RGKY KAAL KADA QD
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
RPMS25, COXPD50, MRP-S25, MRPS25
Positive Sample:
HeLa, A-549, BxPC-3
Cellular Localization:
Mitochondrial Inner Membrane, Mitochondrion.
Calculated MW:
20kDa
Observed MW:
20kDa
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein. A pseudogene corresponding to this gene is found on chromosome 4. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
64432
RRID
AB_2770437
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using MRPS25 Rabbit pAb (CAB9083) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
