CTSF
CTSF (cathepsin F, also known as CATSF and CLN13) is a lysosomal thiol protease of the papain-like peptidase C1 family. It is believed to take part in the intracellular degradation and turnover of proteins. Browse Assay Genie's current range of CTSF research reagents below, or read on for the biology behind the target.
CTSF is a thiol protease that is believed to participate in the intracellular degradation and turnover of proteins. It belongs to the peptidase C1 family and is one of the papain family cysteine proteinases that make up a major component of the lysosomal proteolytic system. It has also been implicated in tumour invasion and metastasis. In UniProt the protein is assigned to the lysosome.
Like other cathepsins, cathepsin F is made with a signal sequence, followed by a propeptide and a catalytically active mature region. The CTSF gene encodes a predicted protein of 484 amino acids that includes a 19-residue signal peptide. Its proregion is very long, at 251 residues. It has a C-terminal domain similar to the pro-segment of cathepsin L-like enzymes, a 50-residue flexible linker peptide, and an N-terminal domain predicted to adopt a cystatin-like fold. This extra segment is unique within the papain family. The cystatin-like domain contains some of the elements known to be important for inhibitory activity, so it resembles the cysteine protease inhibitors of the cystatin superfamily.
Cathepsin F contains five potential N-glycosylation sites. It may reach the endosomal/lysosomal compartment through the mannose 6-phosphate receptor pathway. According to the NCBI Gene summary, the CTSF gene is ubiquitously expressed. It maps to chromosome 11q13, close to the gene encoding cathepsin W. HGNC groups it with the cathepsins, elastases and cysteine endopeptidases, and also lists the aliases CATSF and CLN13.
Cathepsin F is a component of the lysosomal proteolytic system and is believed to contribute to intracellular protein degradation and turnover.
CTSF has been implicated in tumour invasion and metastasis, making it a candidate for studies of protease activity in cancer biology.
The long proregion, with its cystatin-like N-terminal domain, is unique in the papain family and is of interest for studying how cysteine proteases are regulated.
Cathepsin F may be targeted to endosomes and lysosomes through the mannose 6-phosphate receptor pathway, a route studied in lysosomal sorting.
CTSF encodes cathepsin F, a thiol protease believed to participate in the intracellular degradation and turnover of proteins. It is a lysosomal enzyme of the papain-like peptidase C1 family and has also been implicated in tumour invasion and metastasis.
CTSF is the HGNC symbol for cathepsin F. It is also listed under the aliases CATSF and CLN13. The enzyme carries the EC number 3.4.22.41, and HGNC places it in the cathepsin, elastase and cysteine endopeptidase gene groups.
The NCBI Gene summary describes the human CTSF gene as ubiquitously expressed. A mouse ortholog, Ctsf, is recorded. Researchers working with other species should check the datasheet of any product for its validated reactivity.
Other cathepsins and lysosomal proteases that are relevant to protein turnover and protease research.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q9UBX1; product specifications from Assay Genie datasheets.