MCM7
MCM7 (minichromosome maintenance complex component 7, also known as CDC47, PPP1R104 and MCM2) is a subunit of the MCM2-7 complex, the replicative helicase that unwinds template DNA. It is essential for initiating and sustaining DNA replication once per cell cycle in eukaryotic cells. Browse Assay Genie's current range of MCM7 research reagents below, or read on for the biology behind the target.
MCM7 acts as a component of the MCM2-7 complex, the replicative helicase required for 'once per cell cycle' DNA replication initiation and elongation. It is a core component of the CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication and around which the replisome is built. The active ATPase sites in the MCM2-7 ring form at the interface of two neighbouring subunits, so that a conserved arginine finger is provided in trans to the Walker A box ATP-binding site of the adjacent subunit. The six ATPase sites probably contribute differentially to helicase activity. MCM7 is also required for S-phase checkpoint activation after UV-induced damage.
The MCM7 gene encodes one of the highly conserved mini-chromosome maintenance proteins, which belong to the MCM family and are essential for the initiation of eukaryotic genome replication. The hexameric complex is a key component of the pre-replication complex and may be involved in forming replication forks and recruiting other replication-related proteins. A complex of MCM7 with MCM2, MCM4 and MCM6 has DNA helicase activity and may act as a DNA unwinding enzyme. In the nucleus, the protein is found on chromosomes. Alternatively spliced transcript variants encoding distinct isoforms have been reported, and a mouse ortholog, Mcm7, exists.
Cyclin D1-dependent kinase CDK4 associates with MCM7 and may regulate its binding to the tumour suppressor RB1. The gene's earlier names refer to the yeast minichromosome maintenance deficient mutants in which the family was first defined. HGNC also places MCM7 among the protein phosphatase 1 regulatory subunits, the MCM2-7 complex group and the microRNA protein coding host genes.
MCM7 is part of the MCM2-7 helicase required for once-per-cell-cycle replication initiation and elongation, making it central to studies of origin licensing.
As a core component of the CDC45-MCM-GINS helicase, MCM7 is relevant to work on DNA unwinding and replisome assembly.
MCM7 is required for S-phase checkpoint activation after UV-induced damage, linking replication machinery to the DNA damage response.
CDK4 associates with MCM7 and may regulate its binding to RB1, connecting replication proteins to cell cycle control.
MCM7 is a subunit of the MCM2-7 replicative helicase, which is essential for DNA replication initiation and elongation. It is a core part of the CDC45-MCM-GINS helicase that unwinds template DNA, and it is required for S-phase checkpoint activation after UV-induced damage.
MCM7 is also known as CDC47, PPP1R104 and MCM2. Its earlier names were minichromosome maintenance deficient 7 (S. cerevisiae). UniProt lists the protein as DNA replication licensing factor MCM7. Alternatively spliced transcript variants encoding distinct isoforms have been reported.
Yes. NCBI describes the MCM proteins as highly conserved and essential for initiating eukaryotic genome replication. The mouse ortholog is Mcm7. For any MCM7 antibody or MCM7 ELISA kit, check the datasheet of any product for its validated reactivity.
Other MCM2-7 helicase subunits and associated replication factors with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt P33993; product specifications from Assay Genie datasheets.