SENP1
SENP1 (SUMO specific peptidase 1, also known as sentrin-specific protease 1) is a cysteine protease of the peptidase C48 family that acts in the SUMO pathway. It matures SUMO propeptides and removes SUMO from modified proteins. Browse Assay Genie's current range of SENP1 research reagents below, or read on for the biology behind the target.
SENP1 catalyses two essential steps in the SUMO pathway. First, it hydrolyses an alpha-linked peptide bond at the C-terminal end of the SUMO1, SUMO2 and SUMO3 propeptides, producing the mature proteins. Second, it deconjugates SUMO1, SUMO2 and SUMO3 from target proteins by cleaving the epsilon-linked bond between the C-terminal glycine of mature SUMO and the lysine epsilon-amino group of the substrate. Through this second activity, SENP1 reverses sumoylation and regulates the function of many modified proteins.
Reported substrates show how SENP1 acts on different processes. It deconjugates SUMO1 from HIPK2, and from HDAC1 and BHLHE40/DEC1, which decreases their transcriptional repression activity. It removes SUMO1 from CLOCK, which decreases its transcriptional activation activity, and SUMO2 from MTA1. SENP1 also deconjugates SUMO1 from GPS2, and desumoylates CCAR2, which decreases its interaction with SIRT1. In RNA regulation, SENP1 inhibits N(6)-methyladenosine (m6A) RNA methylation by removing SUMO1 from METTL3 and ALKBH5. METTL3 inhibits the m6A RNA methyltransferase activity, while ALKBH5 desumoylation promotes m6A demethylation.
The SENP1 gene encodes a protein found in the nucleus and cytoplasm, and alternate splicing gives multiple transcript variants. HGNC classifies it in the SUMO specific peptidases group, and previous names include SUMO1/sentrin specific protease 1. The mouse ortholog is Senp1. Related family members include SENP2, SENP5 and SENP7.
SENP1 both processes SUMO precursors and deconjugates SUMO1, SUMO2 and SUMO3, making it a central enzyme for studying the SUMO cycle.
SENP1 desumoylates HDAC1, BHLHE40/DEC1 and CLOCK, altering their repression or activation activity.
SENP1 removes SUMO1 from METTL3 and ALKBH5, linking SUMO control to m6A RNA methylation and demethylation.
As a peptidase C48 family cysteine protease, SENP1 is used to compare SUMO paralogue handling with other SUMO specific peptidases.
SENP1 is a SUMO-specific protease. It cleaves SUMO1, SUMO2 and SUMO3 propeptides into mature forms and removes SUMO from modified proteins by cleaving the epsilon-linked bond to lysine. Substrates include HIPK2, HDAC1, CLOCK, MTA1, METTL3 and ALKBH5.
SENP1 stands for SUMO specific peptidase 1, and UniProt calls the protein sentrin-specific protease 1. Earlier HGNC names were SUMO1/sentrin specific protease 1 and SUMO1/sentrin specific peptidase 1. Alternate splicing produces multiple transcript variants.
The mouse ortholog of human SENP1 is Senp1. Human SENP1 belongs to the peptidase C48 family and to the HGNC group of SUMO specific peptidases, which also includes SENP2, SENP5 and SENP7. Check the datasheet of any product for its validated reactivity.
Other SUMO specific peptidases that act in the same pathway, with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q9P0U3; product specifications from Assay Genie datasheets.