FBXO32
FBXO32 (F-box protein 32, also known as MAFbx, ATROGIN1 and Fbx32) is the substrate recognition component of an SCF E3 ubiquitin-protein ligase complex. It directs target proteins for ubiquitination and proteasomal degradation and is closely linked to skeletal muscle atrophy. Browse Assay Genie's current range of FBXO32 research reagents below, or read on for the biology behind the target.
FBXO32 acts as the substrate recognition component of an SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex. In this role it mediates the ubiquitination and subsequent proteasomal degradation of target proteins. It probably recognises and binds phosphorylated targets during skeletal muscle atrophy, and it is reported to recognise TERF1. Beyond muscle, FBXO32 negatively regulates macrophage efferocytosis by promoting ubiquitination of the transcription factor KLF4, which suppresses transcription of the receptor tyrosine kinase MERTK. It also regulates LPS-induced apoptosis and mitochondrial dysfunction through ANXA1 ubiquitination and degradation, and it mediates cyclin D1 stabilisation via 'Lys-27'-linked ubiquitination.
The FBXO32 gene encodes a member of the F-box protein family, defined by an F-box motif of about 40 amino acids. F-box proteins are one of four subunits of SCF complexes, which function in phosphorylation-dependent ubiquitination. They fall into three classes: Fbws with WD-40 domains, Fbls with leucine-rich repeats, and Fbxs with other protein-interaction modules or no recognisable motifs. The FBXO32 protein belongs to the Fbxs class and sits in the HGNC group 'F-boxes other'. UniProt reports it in the cytoplasm and nucleus. Alternative splicing produces multiple transcript variants encoding different isoforms.
FBXO32 is highly expressed during muscle atrophy, and mice lacking the gene are resistant to atrophy. For this reason it is regarded as a potential drug target for muscle wasting. The names MAFbx (muscle atrophy F-box) and atrogin-1 reflect this association, and mouse and rat both carry an Fbxo32 ortholog.
FBXO32 is highly expressed during muscle atrophy, and deficient mice resist atrophy, making it a key readout and potential drug target.
As a substrate receptor in an SCF E3 ligase, FBXO32 is a model for phosphorylation-dependent ubiquitination and proteasomal degradation.
FBXO32 promotes KLF4 ubiquitination, which suppresses MERTK transcription and negatively regulates efferocytosis by macrophages.
FBXO32 links ANXA1 degradation to LPS-induced apoptosis and mitochondrial dysfunction, and stabilises cyclin D1 through Lys-27-linked ubiquitination.
FBXO32 is the substrate recognition component of an SCF E3 ubiquitin-protein ligase complex. It targets proteins for ubiquitination and proteasomal degradation, probably binding phosphorylated substrates during muscle atrophy. Reported substrates include TERF1, KLF4 and ANXA1, and it also stabilises cyclin D1 through Lys-27-linked ubiquitination.
FBXO32 (F-box protein 32) is also called MAFbx, ATROGIN1 and Fbx32, and UniProt lists it as F-box only protein 32. The gene produces multiple transcript variants through alternative splicing, encoding different isoforms. Check the datasheet of any product for its validated reactivity.
Mouse and rat both have an Fbxo32 ortholog. FBXO32 is highly expressed during muscle atrophy, and mice deficient in the gene resist atrophy, so it is considered a potential drug target for muscle wasting. Check the datasheet of any product for its validated reactivity.
Substrates, SCF complex partners and other F-box proteins connected to FBXO32 biology.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q969P5; product specifications from Assay Genie datasheets.