The Fbx32/FBOX32 Monoclonal Antibody (CAB3699) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity and sensitivity for FBX32 in human samples, making it a reliable choice for Western blot applications. By targeting the FBX32 protein, this antibody enables precise detection and analysis in a variety of cell types, making it an essential tool for studies in muscle biology and related fields. FBX32, also known as Atrogin-1, plays a crucial role in the breakdown of muscle proteins, a process that is tightly regulated and critical for muscle maintenance and function.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
Fbx32/FBOX32 Monoclonal Antibody
SKU:
CAB3699
Size:
20μL, 100μL
Reactivity:
Mouse, Rat
Clone Number:
ARC0830
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
Fbx32, MAFbx, Fbx32/FBOX32
Positive Sample:
Mouse skeletal muscle, Rat skeletal muscle
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
42kDa
Observed MW:
42kDa
This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class and contains an F-box domain. This protein is highly expressed during muscle atrophy, whereas mice deficient in this gene were found to be resistant to atrophy. This protein is thus a potential drug target for the treatment of muscle atrophy. Alternative splicing results in multiple transcript variants encoding different isoforms.
Purification Method
Affinity purification
Gene ID
114907
RRID
AB_2863122
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Fbx32/FBXO32 Rabbit mAb (CAB3699) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded Rat heart using Fbx32/FBOX32 Rabbit mAb (CAB3699) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
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