Description
IL1β Rabbit Monoclonal Antibody (CAB27676)
The IL1β Rabbit Monoclonal Antibody (CAB27676) from Assay Genie is a high-quality reagent designed for reliable detection of Interleukin-1 beta (IL-1β) protein in human, mouse, and rat samples. IL-1β is a key pro-inflammatory cytokine produced primarily by activated macrophages and monocytes, playing a central role in the regulation of immune and inflammatory responses, fever induction, and the pathogenesis of numerous inflammatory and autoimmune diseases. This antibody is validated for applications including Western blot (WB), immunofluorescence/immunocytochemistry (IF/ICC), immunohistochemistry on paraffin-embedded tissues (IHC-P), and ELISA. The kit optionally includes Bradford Reagent to enable accurate protein quantification and sample normalization. Manufactured and quality-controlled by Assay Genie, this product supports precise and reproducible immunological research and protein analysis.
| Product Name: | IL1β Rabbit Monoclonal Antibody |
| SKU: | CAB27676 |
| Size: | 100 µL |
| Species: | Rabbit |
| Reactivity: | Human, Mouse, Rat |
| Category: | SMab Recombinant Monoclonal Antibody |
| Conjugate: | Unconjugated |
| Immunogen: | Recombinant protein (or fragment). This information is considered to be commercially sensitive. | ||||||
| Tested Applications: | WBIF/ICCIHC-PELISA | ||||||
| Recommended Dilution: |
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| Synonyms: | Il-1b, IL-1beta, IL1β |
| Positive Sample: | THP-1 cells (LPS-treated), RAW 264.7 cells (LPS-treated), SLE Mouse spleen, SLE Mouse thymus, NR8383 cells (LPS-treated) |
| Cellular Localization: | Cytoplasm, Secreted |
| Calculated MW: | 31 kDa |
| Observed MW: | 31 kDa |
Interleukin-1 beta (IL-1β) is a key pro-inflammatory cytokine encoded by the IL1B gene and produced primarily by activated macrophages, monocytes, and dendritic cells. It is initially synthesized as an inactive 31 kDa precursor (pro-IL-1β) that is cleaved by Caspase-1, following inflammasome activation, into its biologically active 17 kDa mature form. IL-1β signals through the IL-1 receptor (IL-1R) to activate downstream pathways including NF-κB and MAPK, leading to the induction of inflammation, fever, leukocyte recruitment, and the expression of additional pro-inflammatory mediators. IL-1β plays a central role in innate immunity, host defense against pathogens, and the pathogenesis of numerous inflammatory, autoimmune, and metabolic disorders, including rheumatoid arthritis, gout, type 2 diabetes, atherosclerosis, and cryopyrin-associated periodic syndromes (CAPS), making it a key therapeutic target.
| Purification Method | Affinity purification |
| Gene ID | 16176 |
| Uniprot ID | P10749 |
| Isotype | IgG |
| Buffer Information | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
 | Western blot analysis of various lysates using IL1β Rabbit mAb (CAB27676) at 1:4000 dilution incubated at room temperature for 1.5 hours. THP-1 and Raw264.7 cells were treated with LPS (1 µg/mL) at 37°C for 8 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 30 µg per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s. |
 | Immunohistochemistry analysis of paraffin-embedded SLE Mouse spleen and normal Mouse spleen tissue using IL1β Rabbit mAb (CAB27676) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded SLE Mouse spleen tissue using IL1β Rabbit mAb (CAB27676) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded SLE Mouse thymus tissue using IL1β Rabbit mAb (CAB27676) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Raw264.7 and Raw264.7 treated with LPS using IL1β Rabbit mAb (CAB27676) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded THP-1 and THP-1 treated with LPS using IL1β Rabbit mAb (CAB27676) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
 | Confocal imaging of RAW 264.7 cells (treated with BFA and LPS) and RAW 264.7 cells (untreated) using IL1β Rabbit mAb (CAB27676, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x. |
 | Confocal imaging of NR8383 cells (treated with BFA and LPS) and NR8383 cells (untreated) using IL1β Rabbit mAb (CAB27676, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x. |
