PRKACB
PRKACB (protein kinase cAMP-activated catalytic subunit beta, also known as PKACb) is a catalytic subunit of cAMP-dependent protein kinase, or protein kinase A. It is a serine/threonine kinase that mediates cAMP signalling downstream of G protein-coupled receptors. Browse Assay Genie's current range of PRKACB research reagents below, or read on for the biology behind the target.
PRKACB mediates cAMP-dependent signalling triggered when ligands bind G protein-coupled receptors. As a catalytic subunit of protein kinase A (PKA), it phosphorylates serine and threonine residues on its substrates. PKA activation regulates diverse cellular processes, including cell proliferation, the cell cycle and differentiation. It also acts on microtubule dynamics, chromatin condensation and decondensation, nuclear envelope disassembly and reassembly, intracellular transport and ion flux.
The kinase has several defined substrates. It phosphorylates PJA2, which binds and ubiquitinates the regulatory subunits of PKA and leads to their proteolysis. In this way PRKACB regulates the abundance of compartmentalised pools of those regulatory subunits. It also phosphorylates GPKOW, which regulates the ability of GPKOW to bind RNA. In addition, PRKACB acts as a negative regulator of mTORC1 by mediating phosphorylation of RPTOR.
The PRKACB gene encodes a member of the protein kinase superfamily, in the AGC Ser/Thr protein kinase family and the cAMP subfamily. HGNC places it in the protein kinase A subunits and protein kinase A family groups, and also in the centriolar satellite proteins group. Its previous names were protein kinase, cAMP-dependent, catalytic, beta, and protein kinase, cAMP-dependent, beta catalytic subunit. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed. UniProt records the protein in the cytoplasm, cell membrane and nucleus. Mouse and rat both carry a Prkacb ortholog.
PRKACB is a catalytic subunit of PKA and mediates cAMP signalling triggered by receptor binding to G protein-coupled receptors.
PKA activation regulates cell proliferation, the cell cycle and differentiation, processes in which cAMP signalling is important.
PRKACB acts as a negative regulator of mTORC1 by mediating phosphorylation of RPTOR, linking cAMP signalling to mTORC1 control.
PKA activity influences microtubule dynamics, chromatin condensation and decondensation, and nuclear envelope disassembly and reassembly.
PRKACB is a catalytic subunit of cAMP-dependent protein kinase. It mediates cAMP signalling after GPCR activation and phosphorylates substrates such as PJA2, GPKOW and RPTOR. Through these, it influences processes including proliferation, the cell cycle, differentiation, intracellular transport and ion flux.
PRKACB is also called PKACb. Earlier names include protein kinase, cAMP-dependent, catalytic, beta. NCBI Gene reports multiple alternatively spliced transcript variants that encode distinct isoforms, so check which isoform a given reagent is intended to recognise.
Mouse and rat each have a Prkacb ortholog of the human PRKACB gene. The protein belongs to the cAMP subfamily of the AGC Ser/Thr protein kinase family. Check the datasheet of any product for its validated reactivity.
Other protein kinase A subunits, mTORC1 pathway components and PKA substrates with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt P22694; product specifications from Assay Genie datasheets.