Anti-PRKACB Antibody (CAB5324)

Antibody Name:	Anti-PRKACB Antibody
Antibody SKU:	CAB5324
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-351 of human PRKACB (NP_002722.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	SW480, U-87MG, HepG2, mouse heart

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-351 of human PRKACB (NP_002722.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MGNA ATAK KGSE VESV KEFL AKAK EDFL KKWE NPTQ NNAG LEDF ERKK TLGT GSFG RVML VKHK ATEQ YYAM KILD KQKV VKLK QIEH TLNE KRIL QAVN FPFL VRLE YAFK DNSN LYMV MEYV PGGE MFSH LRRI GRFS EPHA RFYA AQIV LTFE YLHS LDLI YRDL KPEN LLID HQGY IQVT DFGF AKRV KGRT WTLC GTPE YLAP EIIL SKGY NKAV DWWA LGVL IYEM AAGY PPFF ADQP IQIY EKIV SGKV RFPS HFSS DLKD LLRN LLQV DLTK RFGN LKNG VSDI KTHK WFAT TDWI AIYQ RKVE APFI PKFR GSGD TSNF DDYE EEDI RVSI TEKC AKEF GEF
Gene ID:	5567
Uniprot:	P22694
Cellular Location:	Cell membrane, Cytoplasm, Lipid-anchor, Membrane, Nucleus
Calculated MW:	29kDa/36kDa/39kDa/40kDa/41kDa/46kDa
Observed MW:	37kDa

Synonyms:

PRKACB, PKA C-beta, PKACB, PKAC-beta

Background:

The protein encoded by this gene is a member of the serine/threonine protein kinase family. The encoded protein is a catalytic subunit of cAMP (cyclic AMP)-dependent protein kinase, which mediates signalling though cAMP. cAMP signaling is important to a number of processes, including cell proliferaton and differentiation. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed.

UniProt Protein Function:	PKACB: Mediates cAMP-dependent signaling triggered by receptor binding to GPCRs. PKA activation regulates diverse cellular processes such as cell proliferation, the cell cycle, differentiation and regulation of microtubule dynamics, chromatin condensation and decondensation, nuclear envelope disassembly and reassembly, as well as regulation of intracellular transport mechanisms and ion flux. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis. A number of inactive tetrameric holoenzymes are produced by the combination of homo- or heterodimers of the different regulatory subunits associated with two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. The cAMP-dependent protein kinase catalytic subunit binds PJA2. Isoform 1 is most abundant in the brain, with low level expression in kidney. Isoform 2 is predominantly expressed in thymus, spleen and kidney. Isoform 3 and isoform 4 are only expressed in the brain. Activated by cAMP. Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cAMP subfamily. 9 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Protein kinase, AGC; EC 2.7.11.11; AGC group; PKA family Chromosomal Location of Human Ortholog: 1p31.1 Cellular Component: nucleoplasm; centrosome; perinuclear region of cytoplasm; plasma membrane; cytosol; cAMP-dependent protein kinase complex Molecular Function:protein binding; cAMP-dependent protein kinase activity; ubiquitin protein ligase binding; magnesium ion binding; ATP binding Biological Process: epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; water transport; activation of protein kinase A; glucose metabolic process; pathogenesis; negative regulation of meiotic cell cycle; signal transduction; protein amino acid phosphorylation; gluconeogenesis; G-protein signaling, coupled to cAMP nucleotide second messenger; synaptic transmission; phospholipase C activation; triacylglycerol catabolic process; carbohydrate metabolic process; neural tube closure; energy reserve metabolic process; renal water homeostasis; innate immune response; blood coagulation; transmembrane transport; regulation of insulin secretion
NCBI Summary:	The protein encoded by this gene is a member of the serine/threonine protein kinase family. The encoded protein is a catalytic subunit of cAMP (cyclic AMP)-dependent protein kinase, which mediates signalling though cAMP. cAMP signaling is important to a number of processes, including cell proliferaton and differentiation. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2014]
UniProt Code:	P22694
NCBI GenInfo Identifier:	125210
NCBI Gene ID:	5567
NCBI Accession:	P22694.2
UniProt Secondary Accession:	P22694,Q14VH1, Q59GC0, Q5BNE9, Q5BNF0, Q5BNF1, Q5BNF2 Q5BNF3, Q5CZ92, B1APG4, B4DKB0, B4E2Q1,
UniProt Related Accession:	P22694
Molecular Weight:	37,110 Da
NCBI Full Name:	cAMP-dependent protein kinase catalytic subunit beta
NCBI Synonym Full Names:	protein kinase, cAMP-dependent, catalytic, beta
NCBI Official Symbol:	PRKACB
NCBI Official Synonym Symbols:	PKACB; PKA C-beta
NCBI Protein Information:	cAMP-dependent protein kinase catalytic subunit beta; protein kinase A catalytic subunit beta; cAMP-dependent protein kinase catalytic beta subunit isoform 4ab
UniProt Protein Name:	cAMP-dependent protein kinase catalytic subunit beta
Protein Family:	cAMP-dependent protein kinase
UniProt Gene Name:	PRKACB
UniProt Entry Name:	KAPCB_HUMAN

	Western blot analysis of extracts of SW480 cells, using PRKACB antibody (CAB5324) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Western blot analysis of extracts of various cell lines, using PRKACB antibody (CAB5324) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Immunohistochemistry of paraffin-embedded rat brain using PRKACB antibody (CAB5324) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human appendix using PRKACB antibody (CAB5324) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of NIH-3T3 cells using PRKACB Rabbit pAb (CAB5324) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-PRKACB Antibody (CAB15709)	Human PRKACB / PKA beta (catalytic subunit) ELISA Kit
Anti-PRKACB Antibody (CAB18067)[KO Validated]	Human cAMP-dependent protein kinase catalytic subunit beta (PRKACB) ELISA Kit
PRKACB Antibody (PACO04710)	KAPCB Colorimetric Cell-Based ELISA
PRKACB Antibody (PACO11466)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)