PFKP
PFKP (phosphofructokinase, platelet; also known as PFK-C or PFKF) is the platelet-type isoform of ATP-dependent 6-phosphofructokinase. It catalyses the first committing step of glycolysis and is studied in glycolytic regulation and cancer metabolism. Browse Assay Genie's current range of PFKP research reagents below, or read on for the biology behind the target.
PFKP is an ATP-dependent 6-phosphofructokinase that catalyses the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate using ATP. This reaction is the first committing step of glycolysis, which makes the enzyme a key point of control over glycolytic flux. The protein is found in the cytoplasm, where glycolysis takes place.
The PFKP gene encodes the platelet-specific isoform of phosphofructokinase, and the encoded enzyme plays a key role in glycolysis regulation. By UniProt classification the protein belongs to the phosphofructokinase type A (PFKA) family, within the ATP-dependent PFK group I subfamily and the eukaryotic two-domain clade 'E' sub-subfamily. HGNC places the gene in the phosphofructokinase family. Alternative splicing of the PFKP gene results in multiple transcript variants. The aliases PFK-C and PFKF are also used in the literature. Orthologous genes are designated Pfkp in mouse and rat.
PFKP has been proposed to contribute to metabolic reprogramming in some cancers, including clear cell renal cell carcinoma and cancers of the bladder, breast and lung. For this reason the enzyme is of interest to researchers studying how tumour cells alter glycolysis. Work on the PFKP protein often looks at its expression and activity alongside other glycolytic enzymes.
PFKP catalyses the first committing step of glycolysis, so it is a natural focus for studies of how glycolytic flux is controlled.
PFKP may contribute to metabolic reprogramming in clear cell renal cell carcinoma and in bladder, breast and lung cancers.
As the platelet-specific isoform of phosphofructokinase, PFKP is used to compare isoform roles within the phosphofructokinase family.
Alternative splicing of the PFKP gene yields multiple transcript variants, which is relevant when designing and interpreting expression studies.
PFKP catalyses the ATP-dependent phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate, the first committing step of glycolysis. It is the platelet-specific isoform of phosphofructokinase and plays a key role in regulating glycolysis. It is located in the cytoplasm.
PFKP stands for phosphofructokinase, platelet. UniProt names the protein ATP-dependent 6-phosphofructokinase, platelet type, and PFK-C and PFKF are listed as aliases. Alternative splicing of the gene results in multiple transcript variants, so check which variant a study addresses.
NCBI Gene reports that PFKP may play a role in metabolic reprogramming in some cancers, including clear cell renal cell carcinoma and cancers of the bladder, breast and lung. Mouse and rat both carry a Pfkp ortholog; check the datasheet of any product for its validated reactivity.
Other phosphofructokinase isoforms, glycolytic enzymes and metabolic markers relevant to cancer metabolism research.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q01813; product specifications from Assay Genie datasheets.