IMPDH2
IMPDH2 (inosine-5'-monophosphate dehydrogenase 2) is the enzyme that catalyses the first committed, rate-limiting step in the de novo synthesis of guanine nucleotides. It converts IMP to XMP and so supports cell growth, DNA synthesis and RNA synthesis. Browse Assay Genie's current range of IMPDH2 research reagents below, or read on for the biology behind the target.
IMPDH2 catalyses the NAD-dependent oxidation of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP). This is the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides. XMP is then converted into guanosine 5'-monophosphate. Through this reaction the enzyme helps maintain the cellular pools of guanine deoxy- and ribonucleotides needed for DNA and RNA synthesis, and it therefore plays an important role in regulating cell growth.
The IMPDH2 gene encodes a member of the IMPDH/GMPR family, classed by HGNC in the inosine monophosphate dehydrogenase family. The protein is found in the cytoplasm, including the cytosol, and in the nucleus. The HGNC name is inosine monophosphate dehydrogenase 2, and an earlier name was IMP (inosine 5'-monophosphate) dehydrogenase 2. Mouse and rat both have an orthologous gene, Impdh2. IMPDH1 is the related family member.
UniProt notes that the protein could also have single-stranded nucleic acid-binding activity and could play a role in RNA and/or DNA metabolism. The IMPDH2 gene is up-regulated in some neoplasms, which suggests a role in malignant transformation. UniProt also reports that the protein may contribute to the development of malignancy and to the growth progression of some tumours. This makes IMPDH2 a frequent subject in studies of nucleotide metabolism and proliferation.
IMPDH2 catalyses the rate-limiting step of de novo guanine nucleotide synthesis, making it a central enzyme for studying purine metabolism.
By maintaining guanine nucleotide pools for DNA and RNA synthesis, IMPDH2 plays an important role in regulating cell growth.
IMPDH2 is up-regulated in some neoplasms and may contribute to malignant transformation and the growth progression of some tumours.
IMPDH2 could bind single-stranded nucleic acids and may play a role in RNA and/or DNA metabolism beyond its catalytic function.
IMPDH2 catalyses the NAD-dependent oxidation of IMP to XMP, the first committed and rate-limiting step in de novo guanine nucleotide synthesis. This supplies the guanine deoxy- and ribonucleotides needed for DNA and RNA synthesis and so influences cell growth. Check the datasheet of any product for its validated reactivity.
The HGNC name is inosine monophosphate dehydrogenase 2, and UniProt lists inosine-5'-monophosphate dehydrogenase 2. A previous name was IMP (inosine 5'-monophosphate) dehydrogenase 2. No alternative gene symbols are listed. The enzyme is classified as EC 1.1.1.205.
NCBI Gene reports that IMPDH2 is up-regulated in some neoplasms, suggesting a role in malignant transformation, and UniProt says it may contribute to the growth progression of some tumours. Mouse and rat each have an ortholog named Impdh2. Check the datasheet of any product for its validated reactivity.
The related IMP dehydrogenase family member and other targets in guanine nucleotide metabolism.
Gene and protein annotations from HGNC, NCBI Gene and UniProt P12268; product specifications from Assay Genie datasheets.