Anti-IMPDH2 Antibody (CAB5350)

Antibody Name:	Anti-IMPDH2 Antibody
Antibody SKU:	CAB5350
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 215-514 of human IMPDH2 (NP_000875.2).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	HeLa, PC-3, Raji, Jurkat, Mouse spleen, Mouse skeletal muscle, Mouse heart, Mouse testis, Mouse trachea

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 215-514 of human IMPDH2 (NP_000875.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	DDEL VAII ARTD LKKN RDYP LASK DAKK QLLC GAAI GTHE DDKY RLDL LAQA GVDV VVLD SSQG NSIF QINM IKYI KDKY PNLQ VIGG NVVT AAQA KNLI DAGV DALR VGMG SGSI CITQ EVLA CGRP QATA VYKV SEYA RRFG VPVI ADGG IQNV GHIA KALA LGAS TVMM GSLL AATT EAPG EYFF SDGI RLKK YRGM GSLD AMDK HLSS QNRY FSEA DKIK VAQG VSGA VQDK GSIH KFVP YLIA GIQH SCQD IGAK SLTQ VRAM MYSG ELKF EKRT SSAQ VEGG VHSL HSYE KRLF
Gene ID:	3615
Uniprot:	P12268
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	55kDa
Observed MW:	56kDa

Synonyms:

IMPDH2, IMPD2, IMPDH-II

Background:

This gene encodes the rate-limiting enzyme in the de novo guanine nucleotide biosynthesis. It is thus involved in maintaining cellular guanine deoxy- and ribonucleotide pools needed for DNA and RNA synthesis. The encoded protein catalyzes the NAD-dependent oxidation of inosine-5'-monophosphate into xanthine-5'-monophosphate, which is then converted into guanosine-5'-monophosphate. This gene is up-regulated in some neoplasms, suggesting it may play a role in malignant transformation.

UniProt Protein Function:	Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth. Could also have a single-stranded nucleic acid-binding activity and could play a role in RNA and/or DNA metabolism. It may also have a role in the development of malignancy and the growth progression of some tumors.
NCBI Summary:	This gene encodes the rate-limiting enzyme in the de novo guanine nucleotide biosynthesis. It is thus involved in maintaining cellular guanine deoxy- and ribonucleotide pools needed for DNA and RNA synthesis. The encoded protein catalyzes the NAD-dependent oxidation of inosine-5'-monophosphate into xanthine-5'-monophosphate, which is then converted into guanosine-5'-monophosphate. This gene is up-regulated in some neoplasms, suggesting it may play a role in malignant transformation. [provided by RefSeq, Jul 2008]
UniProt Code:	P12268
NCBI GenInfo Identifier:	124419
NCBI Gene ID:	3615
NCBI Accession:	P12268.2
UniProt Secondary Accession:	P12268,Q6LEF3,
UniProt Related Accession:	P12268
Molecular Weight:	55,805 Da
NCBI Full Name:	Inosine-5'-monophosphate dehydrogenase 2
NCBI Synonym Full Names:	inosine monophosphate dehydrogenase 2
NCBI Official Symbol:	IMPDH2
NCBI Official Synonym Symbols:	IMPD2; IMPDH-II
NCBI Protein Information:	inosine-5'-monophosphate dehydrogenase 2
UniProt Protein Name:	Inosine-5'-monophosphate dehydrogenase 2
UniProt Synonym Protein Names:	IMPDH-II
UniProt Gene Name:	IMPDH2

	Western blot analysis of extracts of various cell lines, using IMPDH2 antibody (CAB5350) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 20s.
	Immunofluorescence analysis of HeLa cells using IMPDH2 antibody (CAB5350).

Antibodies	ELISA Kits
Anti-IMPDH2 Antibody (CAB15626)	Human IMPDH2 (Inosine 5?-Monophosphate Dehydrogenase 2) ELISA Kit (HUFI08041)
Anti-IMPDH2 Antibody (CAB9208)
IMPDH2 Antibody (PACO09950)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)