AOAH
AOAH (acyloxyacyl hydrolase) is a secreted enzyme that removes the secondary fatty acyl chains from the lipid A region of bacterial lipopolysaccharide. By breaking down LPS, it helps end the host response to Gram-negative bacterial infection. Browse Assay Genie's current range of AOAH research reagents below, or read on for the biology behind the target.
AOAH is a hydrolase that removes the secondary, acyloxyacyl-linked fatty acyl chains from the lipid A region of bacterial lipopolysaccharides (LPS). This effectively detoxifies the LPS molecule. By degrading LPS in this way, the enzyme terminates the host response to bacterial infection and prevents prolonged and damaging inflammatory responses. In peritoneal macrophages, AOAH appears to be important for recovery from a state of immune tolerance following infection by Gram-negative bacteria.
The AOAH gene encodes both the light and heavy subunits of acyloxyacyl hydrolase, and alternatively spliced transcript variants have been described. The enzyme is classified as EC 3.1.1.77 and is found in secreted form and in cytoplasmic vesicles. HGNC places it in the saposin B type domain containing gene group. The mouse ortholog is Aoah.
The enzyme was previously listed under the name acyloxyacyl hydrolase (neutrophil). Because it acts on LPS, AOAH is of interest for understanding how the host modulates its inflammatory response to Gram-negative bacteria, and how that response is switched off once the bacterial stimulus has been processed. Researchers studying the AOAH gene or AOAH protein often do so in the context of LPS signalling and immune tolerance.
AOAH removes secondary acyl chains from lipid A, so it is a key enzyme for studying how bacterial lipopolysaccharide is inactivated.
By degrading LPS, AOAH terminates the host response and prevents prolonged, damaging inflammation, making it relevant to resolution biology.
In peritoneal macrophages, AOAH appears important for recovery from immune tolerance after Gram-negative infection.
AOAH may modulate the host inflammatory response to Gram-negative bacteria, supporting research on infection and innate immunity.
AOAH is an enzyme that removes the secondary acyloxyacyl-linked fatty acyl chains from the lipid A region of bacterial LPS. This detoxifies LPS, terminates the host response to infection and prevents prolonged, damaging inflammation. It also appears to help peritoneal macrophages recover from immune tolerance.
The HGNC name is acyloxyacyl hydrolase, previously listed as acyloxyacyl hydrolase (neutrophil), and no other aliases are recorded. According to NCBI Gene, the locus encodes both the light and heavy subunits of the enzyme, and alternatively spliced transcript variants have been described.
A mouse ortholog, Aoah, is recorded for this gene, so the enzyme can be studied in mouse models of Gram-negative infection and LPS response. Rat ortholog data are not given here. Check the datasheet of any product for its validated reactivity.
Other LPS-sensing proteins, innate immune receptors and inflammation markers with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt P28039; product specifications from Assay Genie datasheets.