NCF1
NCF1 (neutrophil cytosolic factor 1, also known as p47phox, NOXO2, NCF1A and SH3PXD1A) is a 47 kDa cytosolic subunit of the phagocyte NADPH oxidase complex. It helps drive the production of superoxide anion, and mutations in the gene are associated with chronic granulomatous disease. Browse Assay Genie's current range of NCF1 research reagents below, or read on for the biology behind the target.
NCF1 is a subunit of the phagocyte NADPH oxidase complex, which mediates the transfer of electrons from cytosolic NADPH to molecular oxygen to produce the superoxide anion. In the activated complex, electrons pass first from NADPH to flavin adenine dinucleotide (FAD) and then via two heme molecules to oxygen, generating superoxide through an outer-sphere reaction.
NCF1 is one of the cytosolic subunits that assemble with the core oxidase complex to activate it. Assembly takes place at the plasma membrane or the phagosomal membrane. Activation is initiated by phosphorylation-dependent binding of the cytosolic NCF1/p47-phox subunit to the C-terminus of CYBA/p22-phox. This explains why the protein is found both in the cytosol and at membranes. Other cytosolic oxidase factors, such as NCF2 and NCF4, belong to the same oxidase system.
The NCF1 gene encodes a 47 kDa protein, which gives rise to the alias p47phox. HGNC places it in the SH3 domain containing gene group. Its previous name, neutrophil cytosolic factor 1 (47kD, chronic granulomatous disease, autosomal 1), reflects its disease link: mutations in this gene have been associated with chronic granulomatous disease. Mouse and rat both carry an orthologous Ncf1 gene, which allows the NCF1 protein to be studied in rodent models.
NCF1 is part of the phagocyte NADPH oxidase that produces superoxide, making it a focus for studies of neutrophil oxidant generation.
Mutations in the NCF1 gene have been associated with chronic granulomatous disease, so the protein is relevant to studies of this disorder.
Phosphorylation-dependent binding of NCF1 to CYBA/p22-phox initiates complex activation, a key step in oxidase assembly.
As a regulator of superoxide production from NADPH and oxygen, NCF1 is relevant to reactive oxygen species biology.
NCF1 is a cytosolic subunit of the phagocyte NADPH oxidase complex. Following phosphorylation, it binds the C-terminus of CYBA/p22-phox, which initiates assembly and activation of the complex at the plasma or phagosomal membrane, leading to superoxide production from NADPH and oxygen.
NCF1 is also called p47phox, NOXO2, NCF1A and SH3PXD1A. UniProt lists it as neutrophil cytosol factor 1. An earlier HGNC name was neutrophil cytosolic factor 1 (47kD, chronic granulomatous disease, autosomal 1). Check the datasheet of any product for its validated reactivity.
Mutations in NCF1 have been associated with chronic granulomatous disease. Orthologous genes, both named Ncf1, exist in mouse and rat, so rodent models can be used to study the protein. Check the datasheet of any product for its validated reactivity.
Other neutrophil cytosolic factors that work alongside NCF1 in the phagocyte NADPH oxidase complex.
Gene and protein annotations from HGNC, NCBI Gene and UniProt P14598; product specifications from Assay Genie datasheets.