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Cell Biology Antibodies 17 Anti-NCF1 Antibody CAB5143
Key Features

p47phox/NCF1 Rabbit Monoclonal Antibody (CAB5143)

Datasheet MSDS
SKU CAB5143
Product Type Antibody
Antibody Type Monoclonal Antibody
Reactivity Human
Host Species Rabbit
Synonyms CGD1, NCF1A, NOXO2, p47phox, SH3PXD1A, p47phox/NCF1
Research Use Cell Biology
€131 - €457

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Description

system_update_altDatasheetsystem_update_altMSDS

p47phox/NCF1 Monoclonal Antibody (CAB5143)

The p47phox/NCF1 Monoclonal Antibody (CAB5143) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, shows high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the NCF1 protein, this antibody enables precise detection and analysis in various cell types, making it ideal for investigations in the fields of immunology and cancer research.NCF1, also known as neutrophil cytosolic factor 1, plays a crucial role in the activation of immune cells and the production of reactive oxygen species. Its involvement in immune response modulation highlights its importance as a target for research into diseases such as cancer, autoimmune disorders, and chronic inflammatory conditions.

This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.

Product Name:p47phox/NCF1 Monoclonal Antibody
SKU:CAB5143
Size:20μL, 100μL
Reactivity:Human
Clone Number:ARC1206
Conjugate:Unconjugated
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:QRQI KRGA PPRR SSIR NAHS IHQR SRKR LSQD AYRR NSVR FLQQ RRRQ ARPG PQSP GSPL EEER QTQR SKPQ PAVP PRPS ADLI LNRC SEST KRKL ASAV
Tested Applications:WB ELISA
Recommended Dilution:
WB1:500 - 1:1000
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:CGD1, NCF1A, NOXO2, p47phox, SH3PXD1A, p47phox/NCF1
Positive Sample:Raji
Cellular Localization:Cytoplasm, Cytoplasmic Side, Membrane, Peripheral Membrane Protein, Cytosol.
Calculated MW:45kDa
Observed MW:47kDa

The protein encoded by this gene is a 47 kDa cytosolic subunit of neutrophil NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in this gene have been associated with chronic granulomatous disease.

Purification MethodAffinity purification
Gene ID653361
RRIDAB_2863464
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of lysates from Raji cells, using p47phox/NCF1 Rabbit mAb (CAB5143) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
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