The CDC42 Monoclonal Antibody (CAB19028) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody is produced in rabbits and has been validated for use in Western blot applications, allowing for the detection and analysis of CDC42 protein levels in various cell types.CDC42 is known for its role in cytoskeletal dynamics and cell polarity, making it a crucial player in processes like cell motility and adhesion. Dysregulation of CDC42 has been linked to diseases such as cancer, where abnormal cell migration and invasion contribute to tumor progression.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
CDC42 Monoclonal Antibody
SKU:
CAB19028
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Clone Number:
ARC0484
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
The protein encoded by this gene is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc 42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Alternative splicing of this gene results in multiple transcript variants. Pseudogenes of this gene have been identified on chromosomes 3, 4, 5, 7, 8 and 20.
Purification Method
Affinity purification
Gene ID
998
RRID
AB_2862520
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using CDC42 Rabbit mAb (CAB19028) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
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