EIF4E2
EIF4E2 (eukaryotic translation initiation factor 4E family member 2, also known as 4EHP, IF4e and EIF4EL3) is an mRNA cap-binding protein of the eukaryotic initiation factor 4E family. It acts as a negative regulator of translation initiation. Browse Assay Genie's current range of EIF4E2 research reagents below, or read on for the biology behind the target.
EIF4E2 binds the 7-methylguanosine cap found at the 5' end of mRNA. Unlike a factor that promotes translation, it is involved in the negative regulation of translational initiation. It also acts upstream of miRNA-mediated gene silencing, where it contributes to inhibition of translation, and it is involved in the rescue of stalled cytosolic ribosomes. The protein is also reported to bind ubiquitin protein ligases.
The EIF4E2 gene encodes a member of the eukaryotic initiation factor 4E family, and the protein is annotated as eukaryotic translation initiation factor 4E type 2. It is located in the cytosol and in P-bodies, cytoplasmic sites associated with mRNA regulation. The mouse ortholog is Eif4e2, which allows the biology of this gene to be studied in murine systems.
EIF4E2 is also linked to innate immune regulation. It is involved in negative regulation of type I interferon-mediated signalling. In SARS-CoV-2 infection, the interaction of non-structural protein 2 (nsp2) with GIGYF2 enhances GIGYF2 binding to EIF4E2 and increases repression of translation initiation of genes involved in the antiviral innate immune response, such as IFNB1. Earlier names for the gene include eukaryotic translation initiation factor 4E-like 3 (EIF4EL3), and the protein is commonly called 4EHP in the literature. Researchers studying this EIF4E2 gene or EIF4E2 protein often compare it with the canonical cap-binding factor EIF4E.
EIF4E2 binds the mRNA cap and negatively regulates translational initiation, making it a focus for studies of how cap-dependent translation is repressed.
EIF4E2 acts upstream of miRNA-mediated silencing by inhibiting translation, so it is relevant to work on post-transcriptional gene regulation.
EIF4E2 is involved in the rescue of stalled cytosolic ribosomes, linking it to research on translation stalling and its resolution.
EIF4E2 negatively regulates type I interferon signalling and, with GIGYF2, represses IFNB1 translation during SARS-CoV-2 infection.
EIF4E2 binds the 7-methylguanosine mRNA cap and negatively regulates translational initiation. It is also involved in miRNA-mediated gene silencing, the rescue of stalled cytosolic ribosomes and negative regulation of type I interferon-mediated signalling. It is found in the cytosol and in P-bodies.
EIF4E2 is also called 4EHP, IF4e and EIF4EL3. The HGNC full name is eukaryotic translation initiation factor 4E family member 2, and the previous name was eukaryotic translation initiation factor 4E-like 3. UniProt lists the protein as eukaryotic translation initiation factor 4E type 2.
Yes. In SARS-CoV-2 infection, nsp2 interacts with GIGYF2 and enhances GIGYF2 binding to EIF4E2, increasing repression of translation initiation of antiviral innate immune genes such as IFNB1. A mouse ortholog, Eif4e2, exists; check the datasheet of any product for its validated reactivity.
Other eukaryotic translation initiation factors, cap-binding partners and translational regulators with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt O60573; product specifications from Assay Genie datasheets.