CTBP2
CTBP2 (C-terminal binding protein 2, also known as ribeye) is a protein with an NAD+ binding domain related to those of NAD+-dependent 2-hydroxyacid dehydrogenases. The gene produces one isoform that acts as a transcriptional repressor and another that is a major component of synaptic ribbons. Browse Assay Genie's current range of CTBP2 research reagents below, or read on for the biology behind the target.
The CTBP2 gene produces alternative transcripts that encode two distinct proteins. One is a transcriptional repressor. The other isoform is a major component of the specialised synapses known as synaptic ribbons, and UniProt annotates isoform 2 as probably acting as a scaffold for these specialised synapses. This dual role means the same gene contributes to both nuclear gene regulation and synaptic organisation.
Both CTBP2 proteins contain an NAD+ binding domain similar to that found in NAD+-dependent 2-hydroxyacid dehydrogenases, and the protein belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. UniProt lists its subcellular locations as the nucleus and the synapse, consistent with the repressor and synaptic ribbon isoforms respectively. Several transcript variants encoding the two isoforms have been found. The mouse and rat orthologues are both named Ctbp2.
Researchers also know the protein as ribeye, a name linked to its role in synaptic ribbons. The related gene CTBP1 encodes a paralogue of the same name family. According to the NCBI Gene summary, a portion of the 3' untranslated region was originally used to map the gene to chromosome 21q21.3, but similar loci elsewhere in the genome were considered likely, and BLAST analysis places the gene on chromosome 10. HGNC lists CTBP2 among the microRNA protein coding host genes.
One CTBP2 isoform functions as a transcriptional repressor, making the gene relevant to studies of how nuclear corepressors regulate gene expression.
The ribeye isoform is a major component of synaptic ribbons, specialised synapses, and is probably a scaffold for them.
Two distinct proteins arise from alternative transcripts, so CTBP2 is a useful model for comparing nuclear and synaptic roles of one gene.
Both isoforms carry an NAD+ binding domain similar to 2-hydroxyacid dehydrogenases, linking the protein to research on NAD+-binding regulatory proteins.
The CTBP2 gene encodes two proteins. One isoform is a transcriptional repressor, while the other is a major component of synaptic ribbons, specialised synapses, where isoform 2 probably acts as a scaffold. Both contain an NAD+ binding domain similar to NAD+-dependent 2-hydroxyacid dehydrogenases.
Ribeye is an alias for CTBP2 and refers to the isoform that is a major component of synaptic ribbons. The other isoform acts as a transcriptional repressor. Both come from alternative transcripts of the same gene, which has several transcript variants encoding two isoforms.
Yes. Mouse and rat both have an orthologous gene named Ctbp2. The human protein belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. Check the datasheet of any product for its validated reactivity.
Related C-terminal binding protein family members with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt P56545; product specifications from Assay Genie datasheets.