GOLGA2
GOLGA2 (golgin A2), also known as GM130 and golgin-95, is a peripheral membrane protein of the cis-Golgi stack. It maintains Golgi structure, tethers vesicles to the Golgi membrane and contributes to spindle pole assembly during mitosis. Browse Assay Genie's current range of GOLGA2 research reagents below, or read on for the biology behind the target.
GOLGA2 acts as a membrane skeleton that maintains the structure of the Golgi apparatus, and as a vesicle tether that facilitates vesicle fusion to the Golgi membrane. It is required for normal protein transport from the endoplasmic reticulum to the Golgi apparatus and on to the cell membrane. Together with p115/USO1 and STX5, it is involved in vesicle tethering and fusion at the cis-Golgi membrane, which preserves the stacked, inter-connected Golgi architecture. It is also required for Golgi ribbon formation and for glycosylation of membrane and secretory proteins.
GOLGA2 has a central role in mitotic Golgi disassembly. At the onset of mitosis, CDK1 phosphorylates Ser-37, which inhibits the interaction with p115/USO1. This prevents tethering of COPI vesicles and so halts transport through the Golgi during mitosis. The same phosphorylation drives spindle pole assembly: GOLGA2 interacts with importin-alpha through its nuclear localisation signal region and recruits it to Golgi membranes, liberating the spindle assembly factor TPX2. TPX2 then activates AURKA kinase and stimulates local microtubule nucleation, and nascent microtubules are captured by GOLGA2, linking Golgi membranes to the spindle. It also regulates centrosome organisation and, probably by the same mechanism, meiotic spindle pole assembly.
The GOLGA2 gene encodes one of the golgins, a family of proteins localised to the Golgi, and the protein belongs to the GOLGA2 family. It localises to the cis-Golgi network membrane, the endoplasmic reticulum-Golgi intermediate compartment membrane and the spindle pole. NCBI notes that several alternatively spliced transcript variants have been described, although their full-length nature has not been determined. Previous names include golgi autoantigen and golgin subfamily a, 2.
GOLGA2 maintains the stacked cisternae and is required for Golgi ribbon formation, making it a key subject for studying Golgi organisation.
With p115/USO1 and STX5, GOLGA2 tethers vesicles at the cis-Golgi and supports transport from the endoplasmic reticulum to the Golgi.
CDK1 phosphorylation of GOLGA2 triggers Golgi disassembly and, via importin-alpha, TPX2 and AURKA, promotes spindle pole assembly.
GOLGA2 is required for glycosylation of membrane and secretory proteins, linking Golgi integrity to secretory pathway function.
GOLGA2 is a peripheral cis-Golgi membrane protein that maintains Golgi structure and tethers vesicles to the Golgi membrane. It supports endoplasmic reticulum-to-Golgi transport, Golgi ribbon formation and glycosylation, and during mitosis it helps assemble the spindle poles and organise centrosomes.
GOLGA2 is also called GM130 and golgin-95. HGNC lists the approved name as golgin A2, with previous names golgi autoantigen and golgin subfamily a, 2. UniProt names the protein golgin subfamily A member 2. Researchers may therefore find GM130 antibody or GM130 literature under this gene.
Yes. Mouse and rat both have an ortholog named Golga2. The human gene belongs to the golgin group, which encodes Golgi-localised proteins; check the datasheet of any product for its validated reactivity.
Other golgin family members and Golgi-associated targets with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q08379; product specifications from Assay Genie datasheets.