ART1
ART1 (ADP-ribosyltransferase 1) is a GPI-linked enzyme that catalyses the mono-ADP-ribosylation of arginine residues in proteins. It is also known as CD296, ARTC1 and, previously, ART2. Browse Assay Genie's current range of ART1 research reagents below, or read on for the biology behind the target.
ART1 catalyses the ADP-ribosylation of arginine residues in proteins. Mono-ADP-ribosylation is a posttranslational modification, and it is the same modification that is interfered with by a variety of bacterial toxins, including cholera toxin, pertussis toxin and the heat-labile enterotoxins of E. coli. UniProt annotates ART1 as having ADP-ribosyltransferase activity toward GLP1R, and the enzyme is classified as EC 2.4.2.31.
The ART1 protein belongs to the Arg-specific ADP-ribosyltransferase family. Its amino acid sequence consists of predominantly hydrophobic N- and C-terminal regions, a feature characteristic of glycosylphosphatidylinositol (GPI)-anchored proteins, which is reflected in its name as a GPI-linked enzyme. UniProt lists its subcellular location as the sarcoplasmic reticulum membrane. The mouse ortholog is Art1.
The ART1 gene was previously designated ART2, and the protein is also referred to as CD296 and ARTC1. HGNC places it in both the CD molecules group and the ADP-ribosyltransferase family. Related family members with pages at Assay Genie include ART3 and ART4, which are useful comparators when studying arginine-directed ADP-ribosylation.
ART1 catalyses mono-ADP-ribosylation of arginine residues, a posttranslational modification, making it a model for studying this form of protein regulation.
Mono-ADP-ribosylation is interfered with by cholera, pertussis and E. coli heat-labile toxins, linking ART1 research to host-toxin mechanisms.
UniProt records ADP-ribosyltransferase activity of ART1 toward GLP1R, pointing to receptor modification as a research question.
The hydrophobic termini of ART1 are characteristic of GPI-anchored proteins, so it is relevant to studies of membrane-tethered enzymes.
ART1 catalyses the ADP-ribosylation of arginine residues in proteins, a form of mono-ADP-ribosylation that acts as a posttranslational modification. UniProt also records ADP-ribosyltransferase activity toward GLP1R. The enzyme is classified as EC 2.4.2.31 and belongs to the Arg-specific ADP-ribosyltransferase family.
ART1 is the HGNC symbol for ADP-ribosyltransferase 1. The gene was previously designated ART2, and aliases include CD296 and ARTC1. UniProt names the protein GPI-linked NAD(P)(+)--arginine ADP-ribosyltransferase 1. Check these names when comparing literature, as older papers may use ART2.
A mouse ortholog, Art1, is listed for the human gene. ART1 is one member of a wider ADP-ribosyltransferase family that includes ART3 and ART4. Check the datasheet of any product for its validated reactivity before using it with a given species.
Other ADP-ribosyltransferase family members with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt P52961; product specifications from Assay Genie datasheets.