CDC27
CDC27 (cell division cycle 27, also known as APC3, ANAPC3 and NUC2) is a tetratricopeptide repeat-containing subunit of the anaphase-promoting complex/cyclosome (APC/C), an E3 ubiquitin ligase that controls progression through the cell cycle. Browse Assay Genie's current range of CDC27 research reagents below, or read on for the biology behind the target.
CDC27 is a component of the anaphase-promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase. The APC/C mediates ubiquitination and subsequent degradation of target proteins. It mainly forms 'Lys-11'-linked polyubiquitin chains and, to a lesser extent, 'Lys-48'- and 'Lys-63'-linked chains, and it catalyses assembly of branched 'Lys-11'/'Lys-48' ubiquitin chains on its substrates. At the metaphase/anaphase transition the APC/C is activated by CDC20, which targets cyclin B and securin for degradation. The mitotic checkpoint complex (MCC) inhibits the APC/C and delays chromosome segregation.
The CDC27 protein belongs to the APC3/CDC27 family and is one of four APC subunits that contain tetratricopeptide (TPR) repeats, motifs that support protein-protein interactions. The complex is composed of eight protein subunits and is highly conserved in eukaryotic cells. UniProt records CDC27 in the nucleus and on the spindle. The CDC27 gene has mouse and rat orthologs, both named Cdc27, and alternative splicing produces multiple transcript variants. Related pseudogenes have been identified on chromosomes 2, 22 and Y.
CDC27 shows strong similarity to Saccharomyces cerevisiae Cdc27 and to the Schizosaccharomyces pombe nuc2 gene product, which explains the alias NUC2. The APC catalyses formation of cyclin B-ubiquitin conjugates, driving ubiquitin-mediated proteolysis of B-type cyclins. CDC27 has been shown to interact with the mitotic checkpoint proteins Mad2, p55CDC and BUBR1, so it may help control the timing of mitosis.
CDC27 is part of the APC/C, an E3 ubiquitin ligase that controls progression through the cell cycle.
CDC27 interacts with Mad2, p55CDC and BUBR1, making it relevant to studies of mitotic timing and the mitotic checkpoint complex.
The APC/C assembles Lys-11, Lys-48 and Lys-63 linked chains, including branched Lys-11/Lys-48 chains, on its target proteins.
APC/C activation by CDC20 at the metaphase/anaphase transition targets cyclin B and securin for degradation.
CDC27 is a subunit of the anaphase-promoting complex/cyclosome, a cell cycle-regulated E3 ubiquitin ligase. The complex ubiquitinates target proteins for degradation, including cyclin B and securin when activated by CDC20, and so controls progression through mitosis.
CDC27 is also called APC3, ANAPC3 and NUC2, and was previously listed under the symbols D0S1430E and D17S978E. The name NUC2 reflects similarity to the Schizosaccharomyces pombe nuc2 gene product. Alternative splicing of the gene gives multiple transcript variants.
Yes. CDC27 is similar to Saccharomyces cerevisiae Cdc27 and S. pombe nuc2, and the APC is highly conserved in eukaryotic cells. Mouse and rat both have a Cdc27 ortholog. Check the datasheet of any product for its validated reactivity.
Other APC/C subunits and activators, cell division cycle proteins and cell cycle regulators with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt P30260; product specifications from Assay Genie datasheets.