SRGAP3 functions as a GTPase-activating protein (GAP) for the small GTPase RAC1 and perhaps Cdc42, but not for RhoA. GAPs stimulate the intrinsic GTP hydrolysis of small GTPases and so switch them to their inactive state. On this basis SRGAP3 may attenuate RAC1 signalling in neurons. Genome-level annotation predicts that it enables GTPase activator activity and that it is active in the postsynapse.

The SRGAP3 gene encodes a protein that HGNC places in several groups: F-BAR domain containing proteins, the AH/BAR family of Rho GTPase activating proteins, and SH3 domain containing proteins. Together these describe a protein that combines a membrane-associated BAR-type module with a catalytic GAP activity and a protein-interaction domain. The mouse ortholog is Srgap3. Subcellular location is not annotated in UniProt.

Predicted biological roles for SRGAP3 include negative regulation of cell migration, nervous system development and regulation of postsynapse assembly. The protein has carried several names, reflected in its aliases MEGAP, WRP, KIAA0411, ARHGAP14 and SRGAP2; its previous HGNC name was SLIT-ROBO Rho GTPase activating protein 2. Researchers working on Rho GTPase signalling and neuronal development use SRGAP3 antibody, SRGAP3 ELISA kit and SRGAP3 protein reagents to study these functions.