ZEB1
ZEB1 (zinc finger E-box binding homeobox 1, also known as TCF8, AREB6 and Zfhx1a) is a nuclear zinc finger transcription factor that acts mainly as a repressor. It inhibits interleukin-2 expression and drives epithelial-mesenchymal transition by repressing E-cadherin. Browse Assay Genie's current range of ZEB1 research reagents below, or read on for the biology behind the target.
ZEB1 is a transcription factor that represses gene expression by binding the E box motif (5'-CANNTG-3'). It was first characterised for its ability to inhibit interleukin-2 (IL-2) gene expression. It also represses the E-cadherin promoter and induces epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. With the corepressor CTBP1, it represses BCL6 transcription.
Its activity depends on context. ZEB1 can enhance or repress the ATP1A1 promoter, depending on the quantity of cDNA and the cell type. In the absence of TGFB1, it represses COL1A2 transcription by binding the E-box in the upstream enhancer region. It positively regulates neuronal differentiation and, during neurogenesis, represses RCOR1 transcription activation. The ZEB1 protein is nuclear and belongs to the delta-EF1/ZFH-1 C2H2-type zinc-finger family. HGNC places the ZEB1 gene in the C2H2-type zinc finger group and the ZF class homeoboxes group. Mouse and rat both carry a Zeb1 ortholog.
The ZEB1 gene was previously named transcription factor 8 (represses interleukin 2 expression), and the symbols TCF8, AREB6, Zfhx1a, NIL-2-A, BZP, ZEB and Zfhep are also in use. Mutations in the gene are associated with posterior polymorphous corneal dystrophy 3 (PPCD3) and late-onset Fuchs endothelial corneal dystrophy (FECD6). Alternatively spliced transcript variants encoding different isoforms have been described. ZEB2 is a related target for comparative work.
ZEB1 represses the E-cadherin promoter and induces EMT through recruitment of SMARCA4/BRG1, making it a central factor for studying cell state transitions.
ZEB1 binds E-box elements and works with corepressors such as CTBP1, providing a model for repressor mechanisms at target promoters.
ZEB1 positively regulates neuronal differentiation and represses RCOR1 transcription activation during neurogenesis.
Mutations in ZEB1 are associated with posterior polymorphous corneal dystrophy 3 and late-onset Fuchs endothelial corneal dystrophy.
ZEB1 is a transcriptional repressor that binds the E box (5'-CANNTG-3'). It inhibits IL-2 expression, represses the E-cadherin promoter to induce epithelial-mesenchymal transition, and positively regulates neuronal differentiation. Depending on context, it can also enhance promoter activity, as reported for ATP1A1.
ZEB1 is also called TCF8, AREB6, Zfhx1a, Zfhep, NIL-2-A, BZP and ZEB. Its earlier HGNC name was transcription factor 8 (represses interleukin 2 expression). The symbols PPCD3 and FECD6 refer to associated corneal dystrophies. Alternatively spliced transcripts encode different isoforms.
Mutations in ZEB1 are associated with posterior polymorphous corneal dystrophy 3 and late-onset Fuchs endothelial corneal dystrophy. Mouse and rat each have a Zeb1 ortholog. Check the datasheet of any product for its validated reactivity.
A related zinc finger E-box binding homeobox factor with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt P37275; product specifications from Assay Genie datasheets.