HJURP
HJURP (Holliday junction recognition protein) is a centromeric protein that acts as a specific chaperone for the histone H3-like variant CENPA, supporting its incorporation and maintenance at centromeres. It is also known as DKFZp762E1312, URLC9, hFLEG1 and FAKTS. Browse Assay Genie's current range of HJURP research reagents below, or read on for the biology behind the target.
HJURP plays a central role in the incorporation and maintenance of the histone H3-like variant CENPA at centromeres. It acts as a specific chaperone for CENPA and is required for the incorporation of newly synthesised CENPA molecules into nucleosomes at replicated centromeres. In doing so, it helps define the chromatin that marks the centromere.
Mechanistically, HJURP prevents CENPA-H4 tetramerisation and prevents premature DNA binding by the CENPA-H4 tetramer, so CENPA is delivered to chromatin in a controlled manner. UniProt also records that the protein directly binds Holliday junctions, which gave it its name. The NCBI Gene summary describes histone binding and identical protein binding activity, and involvement in CENP-A containing chromatin assembly, chromosome segregation and regulation of protein-containing complex assembly.
In cells, the HJURP protein is found in the nucleolus and at the centromere, and is located at the kinetochore. The human HJURP gene is also catalogued under the aliases DKFZp762E1312, URLC9, hFLEG1 and FAKTS, and a mouse ortholog, Hjurp, is annotated. Researchers studying centromere identity, CENPA deposition and chromosome segregation use HJURP as a key reference point, whether through an HJURP antibody, an HJURP ELISA kit or other reagents.
HJURP is required for incorporating newly synthesised CENPA into nucleosomes at replicated centromeres, making it central to studies of centromere maintenance.
It prevents CENPA-H4 tetramerisation and premature DNA binding by the CENPA-H4 tetramer, offering a model for chaperone-controlled nucleosome assembly.
Annotated as involved in chromosome segregation and located at the kinetochore, HJURP links centromere chromatin to faithful chromosome distribution.
HJURP directly binds Holliday junctions, a property relevant to work on DNA structure recognition and its relationship to centromere function.
HJURP is a specific chaperone for the histone H3-like variant CENPA. It is required for incorporating newly synthesised CENPA into nucleosomes at replicated centromeres, and it prevents CENPA-H4 tetramerisation and premature DNA binding by the CENPA-H4 tetramer.
HJURP stands for Holliday junction recognition protein. Aliases listed for the gene include DKFZp762E1312, URLC9, hFLEG1 and FAKTS. The name reflects the protein's ability to bind Holliday junctions directly, as recorded in UniProt.
A mouse ortholog, Hjurp, is annotated for the human HJURP gene. Check the datasheet of any product for its validated reactivity before using it with a particular species.
Centromere proteins, histone variants and chromosome segregation markers studied alongside HJURP.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q8NCD3; product specifications from Assay Genie datasheets.