CKMT2
CKMT2 (creatine kinase, mitochondrial 2), also known as SMTCK or sarcomeric mitochondrial creatine kinase, is a mitochondrial inner membrane enzyme that reversibly transfers phosphate between ATP and phosphogens such as creatine phosphate. It supports energy transduction in tissues with large, fluctuating energy demands. Browse Assay Genie's current range of CKMT2 research reagents below, or read on for the biology behind the target.
CKMT2 reversibly catalyses the transfer of phosphate between ATP and various phosphogens, for example creatine phosphate. Mitochondrial creatine kinase (MtCK) is responsible for moving high-energy phosphate from mitochondria to the cytosolic carrier, creatine. Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. The enzyme is located at the mitochondrial inner membrane.
The CKMT2 gene encodes creatine kinase S-type, mitochondrial, a member of the ATP:guanido phosphotransferase family and of the creatine kinase isoenzyme family. MtCK exists as two isoenzymes encoded by separate genes: the sarcomeric form (CKMT2) and the ubiquitous form. Sarcomeric MtCK shares 80% homology with the coding exons of ubiquitous MtCK. Unlike the cytosolic creatine kinase isoenzymes, which are exclusively dimeric, mitochondrial creatine kinase occurs in two oligomeric forms: dimers and octamers. Three transcript variants of the CKMT2 gene have been found, all encoding the same protein.
The gene contains sequences homologous to several motifs shared among some nuclear genes encoding mitochondrial proteins. These may be important for the coordinated activation of such genes during mitochondrial biogenesis. The previous HGNC name, creatine kinase, mitochondrial 2 (sarcomeric), reflects the sarcomeric designation, and the symbol SMTCK is still used as an alias. Mouse and rat each have a Ckmt2 ortholog, which makes this a convenient target for comparative studies of mitochondrial energy metabolism.
CKMT2 transfers high-energy phosphate from mitochondria to creatine, making it a key enzyme for studying phosphocreatine-linked energy transduction.
Creatine kinase isoenzymes underpin energy transduction in skeletal muscle and heart, tissues with large, fluctuating energy demands.
Brain and spermatozoa also depend on creatine kinase activity, so CKMT2 is relevant to energy supply in these tissues.
The CKMT2 gene carries motifs shared with nuclear genes for mitochondrial proteins, which may help coordinate their activation during mitochondrial biogenesis.
CKMT2 reversibly catalyses phosphate transfer between ATP and phosphogens such as creatine phosphate. As a mitochondrial creatine kinase, it moves high-energy phosphate from mitochondria to cytosolic creatine, supporting tissues with large, fluctuating energy demands, including skeletal muscle, heart, brain and spermatozoa.
CKMT2 is also called SMTCK, and was previously named creatine kinase, mitochondrial 2 (sarcomeric). It is the sarcomeric MtCK, distinct from the ubiquitous MtCK. Three transcript variants encode the same protein, and the enzyme occurs as dimers and octamers.
Sarcomeric CKMT2 has 80% homology with the coding exons of ubiquitous mitochondrial creatine kinase, which is encoded by a separate gene. Cytosolic creatine kinases are exclusively dimeric, whereas mitochondrial forms also form octamers. Mouse and rat both have a Ckmt2 ortholog; check the datasheet of any product for its validated reactivity.
Other mitochondrial creatine kinase isoenzymes and related energy-metabolism targets with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt P17540; product specifications from Assay Genie datasheets.