ILKAP
ILKAP (integrin-linked kinase-associated serine/threonine phosphatase 2C, also known as PPM1O) is a cytoplasmic protein serine/threonine phosphatase of the PP2C family. It associates selectively with integrin-linked kinase and modulates cell adhesion and growth factor signalling. Browse Assay Genie's current range of ILKAP research reagents below, or read on for the biology behind the target.
ILKAP is a protein serine/threonine phosphatase that selectively associates with integrin-linked kinase (ILK). Through this interaction it regulates the kinase activity of ILK, a regulator of integrin-mediated signalling, and so modulates cell adhesion and growth factor signalling. UniProt annotation describes ILKAP as an inhibitor of the ILK-GSK3B signalling axis, and notes that it may play an important role in inhibiting oncogenic transformation.
The enzyme may also contribute to the regulation of cell cycle progression. The proposed mechanism is dephosphorylation of substrates whose appropriate phosphorylation states may be crucial for cell proliferation. By acting on the ILK-mediated signalling of glycogen synthase kinase 3 beta (GSK3beta), ILKAP is thought to participate in the Wnt signalling pathway. The ILKAP gene encodes a member of the PP2C family, and HGNC places it in the group of Mg2+/Mn2+ dependent protein phosphatases. It is classified under EC 3.1.3.16 and localises to the cytoplasm.
The protein has been recorded under several names, including integrin-linked kinase-associated serine/threonine phosphatase 2C, PPM1O, DKFZP434J2031 and FLJ10181. Mouse and rat both carry an orthologous gene, Ilkap. Researchers studying ILKAP usually focus on integrin signalling, Wnt pathway regulation, phosphatase biology and the control of cell proliferation, where measuring ILKAP protein or ILKAP expression alongside ILK and GSK3B can help to interpret signalling outcomes.
ILKAP associates selectively with integrin-linked kinase and modulates cell adhesion, making it relevant to studies of integrin-mediated signalling.
Through ILK-mediated GSK3beta signalling, ILKAP may participate in the Wnt signalling pathway.
ILKAP may regulate cell cycle progression by dephosphorylating substrates whose phosphorylation states are important for cell proliferation.
By inhibiting the ILK-GSK3B signalling axis, ILKAP may help to restrain oncogenic transformation.
ILKAP is a serine/threonine phosphatase that selectively associates with integrin-linked kinase (ILK) and regulates its kinase activity. It modulates cell adhesion and growth factor signalling, inhibits the ILK-GSK3B axis, and may regulate cell cycle progression through dephosphorylation of its substrates.
ILKAP is also called PPM1O, DKFZP434J2031 and FLJ10181. Its full name is integrin-linked kinase-associated serine/threonine phosphatase 2C, and HGNC lists it as ILK associated serine/threonine phosphatase. It belongs to the PP2C family of Mg2+/Mn2+ dependent protein phosphatases.
Mouse and rat each have an orthologous gene named Ilkap; check the datasheet of any product for its validated reactivity. UniProt notes that ILKAP may play an important role in inhibiting oncogenic transformation, but no specific disease association is established in the source data.
Integrin-linked kinase, GSK3B and other PP2C family phosphatases connected to adhesion and Wnt signalling.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q9H0C8; product specifications from Assay Genie datasheets.