CUX1
CUX1 (cut like homeobox 1) is a nuclear homeodomain DNA-binding protein of the CUT homeobox family that contributes to cell cycle progression at the G1/S transition. It is also known as CDP, CUT, CUX, CASP and CUTL1, among other aliases. Browse Assay Genie's current range of CUX1 research reagents below, or read on for the biology behind the target.
CUX1 plays a role in cell cycle progression, in particular at the G1/S transition. As cells progress into S phase, a fraction of CUX1 molecules is proteolytically processed into N-terminally truncated proteins of 110 kDa. The two forms behave differently on DNA: full-length CUX1 binds only transiently and carries the CCAAT-displacement activity, whereas the shorter CDP/Cux p110 makes a stable interaction with DNA and stimulates expression of genes such as POLA1.
The CUX1 protein is a member of the homeodomain family of DNA-binding proteins and belongs to the CUT homeobox family. The NCBI Gene summary notes that it may regulate gene expression, morphogenesis and differentiation, as well as playing a part in cell cycle progression. Its subcellular location is the nucleus. Several alternatively spliced transcript variants of the CUX1 gene encode different isoforms, which is worth bearing in mind when choosing reagents and interpreting results.
The gene has had many names. HGNC lists the previous names as cut (Drosophila)-like 1 (CCAAT displacement protein) and cut-like 1, CCAAT displacement protein, and the aliases include CDP, CDP1, CUX, CUT, Clox, CDP/Cut, CDP/Cux, Cux/CDP, CASP, GOLIM6 and CUTL1. HGNC places it in both the CUT class homeoboxes and pseudogenes group and the golgins group. Mouse and rat orthologs are both named Cux1.
CUX1 acts at the G1/S transition, making it a relevant protein for studying how cells progress into S phase.
The p110 form binds DNA stably and stimulates genes such as POLA1, while full-length CUX1 shows transient binding and CCAAT-displacement activity.
Cleavage of CUX1 into N-terminally truncated 110 kDa proteins as cells enter S phase offers a model of regulated processing.
CUX1 may regulate gene expression, morphogenesis and differentiation, which makes it a point of interest in developmental biology.
CUX1 is a nuclear homeodomain protein involved in cell cycle progression, particularly at the G1/S transition. It may also regulate gene expression, morphogenesis and differentiation. Full-length CUX1 binds DNA transiently with CCAAT-displacement activity, while the processed p110 form binds stably and stimulates genes such as POLA1.
The names reflect its history: CDP (CCAAT displacement protein), CUT and CUTL1 derive from the Drosophila cut gene, while CASP and GOLIM6 are further aliases. Several alternatively spliced transcript variants encode different isoforms, and proteolytic processing in S phase generates a 110 kDa form called CDP/Cux p110.
Yes. Mouse and rat both have an ortholog named Cux1, and the human gene is a member of the CUT homeobox family. Check the datasheet of any product for its validated reactivity before using it with a particular species.
Other homeobox proteins, cell cycle regulators and DNA replication targets related to CUX1 biology.
Gene and protein annotations from HGNC, NCBI Gene and UniProt P39880; product specifications from Assay Genie datasheets.