EDF1
EDF1 (endothelial differentiation related factor 1, also known as EDF-1 and CFAP280) is a transcriptional coactivator found in the cytoplasm and nucleus. It links general and gene-specific transcription machinery and is implicated in endothelial differentiation, lipid metabolism and cardiomyocyte hypertrophy. Browse Assay Genie's current range of EDF1 research reagents below, or read on for the biology behind the target.
EDF1 acts as a transcriptional coactivator. According to UniProt, it stimulates the transcriptional activity of NR5A1, and of ligand-dependent NR1H3 (LXRA) and PPARG. It also enhances the DNA-binding activity of ATF1, ATF2, CREB1 and NR5A1. The NCBI Gene summary describes a complementary mechanism in which the protein interconnects the general transcription factor TATA element-binding protein (TBP) with gene-specific activators.
Outside the nucleus, EDF1 is reported to regulate nitric oxide synthase activity, probably by sequestering calmodulin in the cytoplasm. This fits its localisation to both the cytoplasm and the nucleus. The protein may also function in endothelial cell differentiation, hormone-induced cardiomyocyte hypertrophy and lipid metabolism, so it is of interest to researchers studying vascular biology, cardiac growth responses and metabolic gene regulation.
The human EDF1 gene encodes the protein, and alternate splicing gives rise to multiple transcript variants. Mouse and rat both carry an Edf1 ortholog. The name reflects the early association with endothelial differentiation, while the alias CFAP280 reflects its placement by HGNC in the cilia and flagella associated gene group. Studies of EDF1 typically use an EDF1 antibody, EDF1 ELISA kit or recombinant EDF1 protein to examine expression and function.
EDF1 enhances activity of NR5A1, LXRA and PPARG and bridges TBP with gene-specific activators, making it a useful model of coactivator function.
EDF1 may regulate endothelial cell differentiation, which is the basis of its name and of interest in vascular biology.
EDF1 may contribute to hormone-induced cardiomyocyte hypertrophy, relevant to studies of cardiac growth signalling.
EDF1 may influence lipid metabolism and regulates nitric oxide synthase activity, probably by sequestering calmodulin in the cytoplasm.
EDF1 is a transcriptional coactivator. It stimulates NR5A1, LXRA and PPARG activity, enhances DNA binding by ATF1, ATF2, CREB1 and NR5A1, and connects TBP to gene-specific activators. In the cytoplasm it probably sequesters calmodulin, thereby regulating nitric oxide synthase activity.
EDF1 stands for endothelial differentiation related factor 1 and is also written EDF-1. The gene has the alias CFAP280 and belongs to the HGNC cilia and flagella associated group. NCBI notes that alternate splicing produces multiple transcript variants.
Yes. Mouse and rat each have an ortholog named Edf1, so the protein can be studied in common rodent models. Reactivity differs between reagents, so check the datasheet of any product for its validated reactivity.
Nuclear receptors, CREB/ATF transcription factors and other regulators connected with EDF1 function.
Gene and protein annotations from HGNC, NCBI Gene and UniProt O60869; product specifications from Assay Genie datasheets.