MYOF
MYOF (myoferlin, also known as KIAA1207 or FER1L3) is a calcium- and phospholipid-binding member of the ferlin family. It contributes to plasmalemma repair in endothelial cells, to endocytic recycling and to VEGF signal transduction. Browse Assay Genie's current range of MYOF research reagents below, or read on for the biology behind the target.
MYOF is a calcium/phospholipid-binding protein that plays a role in the plasmalemma repair mechanism of endothelial cells. This mechanism permits rapid resealing of membranes that have been disrupted by mechanical stress. MYOF is also involved in endocytic recycling, and it is implicated in VEGF signal transduction, where it regulates the levels of the receptor KDR.
The myoferlin protein is a type II membrane protein and a member of the ferlin family. According to UniProt it localises to the cell membrane, the nuclear membrane, cytoplasmic vesicle membranes and late endosome membranes, consistent with the RefSeq summary that it associates with both plasma and nuclear membranes. It contains C2 domains, which play a role in calcium-mediated membrane fusion events. This suggests a function in membrane regeneration and repair. Two transcript variants encoding different isoforms of the MYOF gene have been described, and other possible variants have been detected whose full-length nature has not been determined.
MYOF is structurally similar to dysferlin, another ferlin family protein associated with the plasma membrane. Mutations in dysferlin can cause muscle weakness affecting both proximal and distal muscles, which places the ferlins in the study of membrane repair. The gene symbol reflects the original HGNC name, fer-1-like 3 (myoferlin), after the C. elegans fer-1 gene; KIAA1207 and FER1L3 are alternative designations. The mouse ortholog is Myof.
MYOF supports resealing of endothelial plasma membranes disrupted by mechanical stress, making it a focus for studies of membrane regeneration.
MYOF is involved in endocytic recycling and is found on cytoplasmic vesicle and late endosome membranes.
MYOF regulates levels of the receptor KDR, linking it to VEGF signal transduction in endothelial cells.
As a dysferlin-like protein with C2 domains, MYOF is used to explore calcium-mediated membrane fusion across the ferlin family.
MYOF is a calcium/phospholipid-binding protein that takes part in plasmalemma repair in endothelial cells, allowing rapid resealing of membranes disrupted by mechanical stress. It is also involved in endocytic recycling and in VEGF signal transduction, where it regulates levels of the receptor KDR.
MYOF is the symbol for myoferlin, also called KIAA1207 or FER1L3. Earlier HGNC names were fer-1 (C.elegans)-like 3 (myoferlin). RefSeq reports two transcript variants encoding different isoforms, and other possible variants have been detected but not fully characterised.
MYOF is a ferlin family member that is structurally similar to dysferlin, whose mutations can cause muscle weakness. Both carry C2 domains linked to calcium-mediated membrane fusion. The mouse ortholog is Myof; check the datasheet of any product for its validated reactivity.
Other ferlin family members and VEGF signalling partners relevant to membrane repair and endocytic recycling research.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q9NZM1; product specifications from Assay Genie datasheets.