NSD3
NSD3, also known as WHSC1L1, WHISTLE and KMT3F, is a nuclear histone-lysine methyltransferase that preferentially dimethylates histone H3 at lysine 4 and lysine 27. It is a PWWP domain-containing member of the SET2 subfamily and is studied in chromatin and transcriptional regulation. Browse Assay Genie's current range of NSD3 research reagents below, or read on for the biology behind the target.
NSD3 is a histone methyltransferase. It preferentially dimethylates histone H3 at lysine 4 and lysine 27, producing H3K4me2 and H3K27me2. These two marks have different roles: methylation of H3 lysine 4 is a specific tag for epigenetic transcriptional activation, while lysine 27 is a mark for transcriptional repression. The NCBI Gene summary describes the combined methylation of lysines 4 and 27 by the protein as repressing gene transcription. The protein localises to the nucleus and chromosomes, consistent with its role in modifying chromatin.
The NSD3 gene encodes a protein that carries PWWP (proline-tryptophan-tryptophan-proline) domains. HGNC places it in several groups: PHD finger proteins, histone lysine methyltransferases, PWWP domain containing and SET domain containing. UniProt assigns it to the class V-like SAM-binding methyltransferase superfamily, the histone-lysine methyltransferase family and the SET2 subfamily. Two alternatively spliced variants have been described. The mouse ortholog is Nsd3.
The gene is related to the Wolf-Hirschhorn syndrome candidate-1 gene, which explains the previous name Wolf-Hirschhorn syndrome candidate 1-like 1 and the alias WHSC1L1. Other aliases include FLJ20353, WHISTLE and KMT3F. The official name, nuclear receptor binding SET domain protein 3, reflects its SET catalytic domain. Researchers working on the NSD3 protein often use it to study how histone H3 methylation shapes gene expression.
NSD3 writes H3K4me2 and H3K27me2 marks, making it a useful enzyme for studying histone lysine methylation and chromatin state.
Methylation of H3 lysines 4 and 27 by NSD3 is linked to control of gene transcription, activating or repressing depending on the mark.
As a SET2 subfamily, PWWP domain-containing enzyme, NSD3 is a reference point for comparing SET domain methyltransferases and their substrate specificity.
NSD3 is related to the Wolf-Hirschhorn syndrome candidate-1 gene, so it features in studies of this gene family.
NSD3 is a histone methyltransferase that preferentially dimethylates histone H3 at lysine 4 and lysine 27, forming H3K4me2 and H3K27me2. H3 lysine 4 methylation tags transcriptional activation, while lysine 27 marks repression. The NCBI Gene summary notes that this methylation represses gene transcription.
NSD3 is also called WHSC1L1, WHISTLE, KMT3F and FLJ20353. Its earlier name was Wolf-Hirschhorn syndrome candidate 1-like 1. The HGNC name is nuclear receptor binding SET domain protein 3. Two alternatively spliced variants have been described for the gene.
NSD3 belongs to the SET2 subfamily of histone-lysine methyltransferases and is related to the Wolf-Hirschhorn syndrome candidate-1 gene. It has a mouse ortholog, Nsd3. Check the datasheet of any product for its validated reactivity.
Other nuclear and metabolic enzymes with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q9BZ95; product specifications from Assay Genie datasheets.