POU2F1
POU2F1 (POU class 2 homeobox 1, also known as OCT1 or OTF1) is a nuclear transcription factor of the POU family, class-2 subfamily. It binds DNA through its POU domain and is also co-opted by herpes simplex virus during infection. Browse Assay Genie's current range of POU2F1 research reagents below, or read on for the biology behind the target.
POU2F1 is a sequence-specific DNA-binding transcription factor that localises to the nucleus. Like other members of the POU family, it carries the POU domain, a region of about 160 amino acids that is necessary for binding to the octameric sequence ATGCAAAT. This motif gives the protein its common name, OCT1, short for octamer-binding transcription factor 1.
The POU2F1 gene encodes a protein belonging to the class-2 subfamily of POU transcription factors. HGNC places it in the group of POU class homeoboxes and pseudogenes. OCT1 was among the first identified members of the POU family, and work on it helped define the family as a whole. The older name OTF1 and the previous HGNC name, POU domain class 2, transcription factor 1, are still found in the literature. Mouse and rat both have an orthologous Pou2f1 gene, so the POU2F1 gene can be studied in common rodent models.
A well-documented role comes from herpes simplex virus (HSV) infection. In that setting, POU2F1 forms a multiprotein-DNA complex with the viral transactivator protein VP16 and HCFC1. This complex enables transcription of the viral immediate early genes. That makes POU2F1 a useful host factor for studying how viruses use cellular transcription machinery. Researchers working with a POU2F1 antibody, a POU2F1 protein or a POU2F1 ELISA kit often do so in the context of transcriptional regulation, DNA recognition by POU proteins or host-virus interaction. Related POU factors, such as POU2F2 and its cofactor POU2AF1, are useful for comparison.
POU2F1 (OCT1) is a founding member of the POU family and binds the octameric sequence ATGCAAAT through its POU domain.
POU2F1 assembles with VP16 and HCFC1 to enable transcription of HSV immediate early genes during infection.
The roughly 160-amino-acid POU domain is necessary for DNA binding, making POU2F1 a reference for POU protein–DNA recognition.
As a class-2 POU factor, POU2F1 supports comparative work across the POU family and its related subfamilies.
POU2F1 is a nuclear transcription factor that binds DNA through its POU domain, which recognises the octameric sequence ATGCAAAT. During herpes simplex virus infection, it forms a complex with VP16 and HCFC1 that enables transcription of the viral immediate early genes.
OCT1 refers to the octamer-binding activity of the protein, which recognises the ATGCAAAT motif. OTF1 is another alias, and POU domain class 2, transcription factor 1 is a previous HGNC name. All of these names refer to the same POU2F1 gene and protein.
Yes. Mouse and rat each have an ortholog named Pou2f1, so the gene can be studied in rodent models. POU2F1 belongs to the class-2 POU subfamily. Check the datasheet of any product for its validated reactivity.
Other POU family transcription factors and cofactors with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt P14859; product specifications from Assay Genie datasheets.