DDX39B
DDX39B is a DEAD-box RNA-dependent ATPase that acts in pre-mRNA splicing and in the export of mRNA from the nucleus to the cytoplasm. It is also known as Uap56, BAT1 and D6S81E, and was previously named HLA-B associated transcript 1. Browse Assay Genie's current range of DDX39B research reagents below, or read on for the biology behind the target.
DDX39B is a member of the DEAD-box family of RNA-dependent ATPases, which mediate ATP hydrolysis during pre-mRNA splicing. The protein is an essential splicing factor required for the association of U2 small nuclear ribonucleoprotein (snRNP) with pre-mRNA, and it also plays an important role in exporting mRNA from the nucleus to the cytoplasm. In keeping with these roles, UniProt records it in the nucleus, including nuclear speckles, and in the cytoplasm.
UniProt classifies the protein as belonging to the DEAD box helicase family, DECD subfamily, and annotates it as an RNA helicase with the EC number 3.6.4.13. HGNC places the DDX39B gene in the groups of DEAD-box helicases, transcription and export complex 1 subunits, and spliceosomal E complex. The TREX complex, in which it participates, is essential for the export of intronless mRNAs of Kaposi's sarcoma-associated herpesvirus (KSHV) and for production of infectious virus.
The gene was previously named HLA-B associated transcript 1 (BAT1) because it lies in a cluster of genes near those encoding tumour necrosis factor alpha and tumour necrosis factor beta, within the human major histocompatibility complex class III region. Mutations in this gene may be associated with rheumatoid arthritis. Alternative splicing produces multiple transcript variants, related pseudogenes exist on chromosomes 6 and 11, and read-through transcription occurs between this gene and the upstream ATP6V1G2 gene. Mouse and rat orthologs are both named Ddx39b.
DDX39B is required for U2 snRNP association with pre-mRNA, making it a key factor for studying early spliceosome assembly.
DDX39B contributes to export of mRNA from the nucleus to the cytoplasm, a step studied through the TREX complex.
The TREX complex is essential for export of KSHV intronless mRNAs and infectious virus production, linking DDX39B to host-virus research.
The gene sits in the MHC class III region and mutations may be associated with rheumatoid arthritis, supporting genetic and immunological studies.
DDX39B is a DEAD-box RNA-dependent ATPase that mediates ATP hydrolysis during pre-mRNA splicing. It is required for U2 snRNP association with pre-mRNA and also has an important role in exporting mRNA from the nucleus to the cytoplasm.
DDX39B is also known as Uap56, BAT1 and D6S81E. Earlier names include HLA-B associated transcript 1 and DEAD (Asp-Glu-Ala-Asp) box polypeptide 39B. The current HGNC name is DExD-box helicase 39B, and UniProt lists it as spliceosome RNA helicase DDX39B.
Orthologs named Ddx39b exist in mouse and rat, so rodent models can be used for functional work; check the datasheet of any product for its validated reactivity. NCBI Gene notes that mutations in human DDX39B may be associated with rheumatoid arthritis.
Other DEAD-box RNA helicases involved in RNA processing, splicing and export, with products at Assay Genie.
Gene and protein annotations from HGNC, NCBI Gene and UniProt Q13838; product specifications from Assay Genie datasheets.